Subhash Das
Biomedical Research
Edmonton,Canada
Summary
Skilled translational cardiovascular scientist, over 10 years of experience with strategic project planning, complex analysis and interpretation, project management and execution, excellent communication, and effective team player.
Overview
16
16
years of professional experience
Work History
Postdoctoral fellow
Department of Medicine, University of Alberta
11.2019 - Current
- Investigated role of cytoskeletal remodeling in EMT and role of novel cytoskeletal protein CRMP2A by using human stem cells and human cancer cells
- Establishing drug screening platform to study cardiotoxicity by human iPSC derived cardiomyocytes.
Postdoctoral Associate
Yale school of Medicine
10.2016 - 07.2019
- Investigated Inherited heart disease by using patient-specific induced pluripotent stem cells (hiPSCs).
PhD Graduate Studies
University Of Alberta
01.2011 - 07.2016
- Investigated genetic and non-genetic iron-overload cardiomyopathy and liver disease, Resveratrol as potential therapy.
- Generated novel mouse models of iron overload cardiomyopathy and different functional assays like assessment of cardiac function, invitro cell culture,histopathology, imaging techniques, gene expression analysis, WB, data analysis.
Lecturer
Department Of Biotechnology, Aditya Degree College
09.2007 - 12.2009
- Taught undergraduate Biotechnology cources(Molecular Biology/genetics and cell biology) students and conducted exams.
Education
Ph.D. - Experimental Medicine
University of Alberta, Canada
Department Of Medicine07.2016
Master of Science - Biotechnology
Berhampur University, India
Gandhi Institute Of Biological Science08.2007
Bachelor of Science - Biological Sciences
Berhampur University, India
Khallikote Auto. College02.2005
Skills
- In vitro skills:
- Advanced tissue culture techniques, such as Human iPSCs culture and maintenance, Differentiation of iPSCs into cardiomyocytes, smooth muscle cells and disease modeling
- Construction of Engineered Heart Tissues (EHTs) by using patient iPSC derived cardiomyocytes Functional measurements and characterization
- Assay Development: Drug screening plat form,
- Cryopreservation: human iPSCs, iPSCs derived cardiomyocytes and other primary cells and cell lines
- Basic Molecular Biology techniques: DNA/RNA isolation and analysis, molecular cloning, genotyping, karyotyping; rtPCR,ddPCR
- In vivo skills:
- Different murine models of heart failure, cardiotoxicity Tumor xenograft models
- Tissue processing, histopathology and molecular signaling work assessment
- Hematological Analysis: processed blood and tissues for iron parameter analysis
- Human explant and tissue biopsy work: human tissue biopsy handling and processing for work
- DNA sequencing and analysis: Prepare samples for DNA sequencing and analysis from genetic mutation human samples Expertise in sequencing primer design
- Training
- Safety Training: WHMIS, Laboratory Safety, Chemical Safety, Concepts in Biosafety from University of Alberta, Alberta, Canada
- Safety Training: WHMIS, Laboratory Safety, Chemical Safety, Concepts in Biosafety from Yale University, New Haven, CT, USA
- Stem cell Training: Human iPSCs maintenance and differentiation into cardiomyocytes and SMC at Yale stem cell center, Yale University
- Computer skills:
- Microsoft office, statistical analysis software GraphPad Prism, other software like Primer, siRNA, shRNA designing
Personal Information
Citizenship: India
Professional Link
https://www.linkedin.com/in/subhash-k-das-8923b6163/
Publications
Follow this link to see the full list of publication.
https://scholar.google.com/citations?pli=1&user=91lDtbAAAAAJ (Complete List of Publications)
Languages
English
Full Professional
Hindi
Native or Bilingual
Telugu
Native or Bilingual
Oriya
Native or Bilingual
Timeline
Postdoctoral fellow
Department of Medicine, University of Alberta
11.2019 - Current
Postdoctoral Associate
Yale school of Medicine
10.2016 - 07.2019
PhD Graduate Studies
University Of Alberta
01.2011 - 07.2016
Lecturer
Department Of Biotechnology, Aditya Degree College
09.2007 - 12.2009
Ph.D. - Experimental Medicine
University of Alberta, Canada
Master of Science - Biotechnology
Berhampur University, India
Bachelor of Science - Biological Sciences
Berhampur University, India
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