Gunjan Dadhwal

• Molecular biology techniques

RNA and DNA extraction and purification, agarose gel electrophoresis, native gel electrophoresis, denaturing Polyacrylamide gel electrophoresis (PAGE), Southern Blotting, radiolabelling, PCR (R-PCR, qPCR, sequencing PCR, multiplex PCR, LR PCR, tetra-primer ARMS PCR), Sanger sequencing, cDNA synthesis, Site-Directed Mutagenesis, cloning and transformation strategies (TA cloning, Gibson assembly, Golden Gate), knock out construct generation (Cre/loxP conditional knockout), and luciferase assay system

• Biochemistry techniques

Total protein extraction, SDS-PAGE, CE-SDS, 2D electrophoresis, Protein expression and purification (affinity purification, ion-exchange chromatography, gel filtration or size-exclusion chromatography, RP-HPLC, and FPLC/AKTA), cIEF, HIC, DSC, DSF, western blotting, biochemical analysis (protein estimation, enzymatic assay, SEC-MALs, SAXS), bioconjugation, UV-Vis spectrophotometry, CD spectroscopy, RNA-protein interaction studies (EMSA, filter binding assay, SPR), and bioassays

• Cell culture techniques

In vitro cell culture, Maintenance of cell lines (both adherent and suspension) such as HEK-293, Hep G2/Panc1, HeLa, cell counting (manual and automated), cryopreservation, cell plating, microscopy, transfection, and purification of recombinant proteins from bacterial and mammalian expression systems Proficient in optimizing cell culture conditions and protocols to improve cell viability and growth rates

• Bioinformatics

Basic database management, Sequence analysis (pairwise/multiple sequence alignment), Gene Prediction and analysis of gene expression (ENCODE/Ensembl), Prediction of transcription factor binding sites (sTRAP, Genomatix,), BioGrid, and secondary structural prediction tools (mFold, Jpred, Rosetta, etc)

• Immunological techniques

Immunoblotting, Antibody purification, immunoassays such as Enzyme-linked immunosorbent assays (ELISA)

• Computer Skills

Proficient in using Windows, Macintosh OS, and software like CodonCode aligner, ApE, Snapgene, Gentle, Pymol, Adobe Illustrator & Photoshop, GraphPad Prism, Origin Pro, Excel, MS word, powerpoint, R, maintain electronic lab books using OneNote, etc