Hamid Goodarzi

Biomaterials and Ophthalmic Formulations in Dr. May Griffith's Lab

1-CLP/PEG Implants for Corneal Regeneration of HSV-1-Infected Rabbit and Guinea Pig Models:
a. Fabricated implants under ISO 7 cleanroom GMP conditions, including processes such as dissolving, dialysis, lyophilizing, mixing, molding, demolding, and storing in buffers (PBS/Chloroform) for sterility.

b. Conducted comprehensive physico-chemical and optical characterization to ensure material performance and biocompatibility.

2- CLP-PEG-MPC implants for Corneal Regrowth of Rabbit Models:
a. Fabricated and optimized biosynthetic implants under ISO 7 cleanroom GMP conditions.
b. Performed mechanical testing using rheology machines to evaluate elasticity, compression, and tensile properties.
c. Collaborated with ophthalmologists to test the suture tolerance of implants.
d. Conducted stability studies of the implants under varying temperatures to determine optimal storage conditions.

3-Sterilization and Efficacy Testing of CLP-PEG-MPC Implants:
a. Directed implant preparation and sterilization using Electron Beam Irradiation (15kGy) and supercritical CO₂ (scCO₂) methods.
b. Tested the implants’ refractive index, light transmission, backscattering properties, collagenase degradation, contact angle, water content, SEM, FTIR, NMR, and biocompatibility with human corneal and stromal cells.
c. Evaluated efficiency of sterilization methods of the implants against Gram (+) Staphylococcus aureus and Gram (-) Pseudomonas aeruginosa.

Laser Bioprinting for Ophthalmological Applications in Dr. Christos Boutopoulos's Lab (Ph.D. project)

1-In-vivo Bioprinting of Photocrosslinkable Bioinks for Cornea Regeneration:

a. Developed and optimized photocrosslinkable bioinks tailored for corneal regeneration.
b. Conducted printability studies to evaluate bioink performance
c. Assessed whether the laser-assisted bioprinting method alters the physico-chemical and biological properties of the bioinks.
d. Performed preclinical evaluations in mouse models to ensure safety, compatibility, and regenerative efficacy.

1-Fabrication and Characterization of Collagen–Gelatin Hydrogel for Corneal Tissue Engineering

a. Developed gelatin/collagen-based hydrogels cross-linked with EDC/NHS.

b. Characterized physicochemical, mechanical, and optical properties, including water content, enzymatic degradation, rheology, and contact angle.

c. Assessed biocompatibility using hBM-MSCs viability and morphology via CCK-8 assay and phase-contrast microscopy.

d. Published this work in highly impact factor journal with 180 citations so far