Bohan Wei
Lethbridge,Canada
Summary
Detail-oriented researcher with expertise in molecular biology, bioinformatics, and plant pathology. Proficient in PCR, DNA extraction, QTL mapping, and GWAS analysis, with strong skills in statistical analysis using R and programming in Python, Perl, and Java. Experienced in designing and executing experiments related to plant disease diagnosis and pathogen virulence, particularly in cereal crops. Adept at data analysis, protocol development, and managing research projects from inception to completion. Passionate about advancing agricultural science through cutting-edge molecular techniques and collaborative problem-solving.
Overview
6
6
years of professional experience
1
1
Certification
Work History
Research Affiliate
Agriculture and Agri-Food Canada, AAFC
12.2020 - Current
Research on Stripe Rust in Wheat: Conducted field surveys and collected Puccinia striiformis (Pst) isolates from wheat, barley, and foxtail barley across Canada. Characterized virulence using differential wheat and barley lines. Extracted high-quality DNA from urediniospores for genome sequencing and SNP analysis. Investigated fungicide tolerance by amplifying and sequencing the CYP51 gene. Performed genome-wide association studies (GWAS) to identify resistance markers in Canadian wheat panels. Results contribute to understanding virulence patterns, fungicide tolerance, and resistance in stripe rust populations in Canada.
Research Participant
Agriculture and Agri-Food Canada, AAFC
05.2018 - 08.2020
Research on Tan Spot Disease of Wheat: Investigated the race structure of Pyrenophora tritici-repentis (Ptr) across different hosts, including durum, winter bread wheat, and grasses in western Canada. Collected and classified 144 fungal isolates by race, using bioassays and PCR analysis for effector genes (ToxA, ToxB). Evaluated susceptibility of 114 wheat genotypes and explored the genetic basis of Ptr-barley interaction using doubled-haploid barley populations. Mapped a locus controlling chlorosis (Spr1) on barley chromosome 2H. Findings contribute to understanding host-pathogen dynamics and improving disease management.
Education
Ph.D. - Plant Science
University of Alberta
Edmonton, AB04.2025
Master of Science - Plant Science
University of Alberta
Edmonton, AB08.2020
Bachelor of Science - Forestry
Northeast Forestry University
Harbin, China07.2017
Skills
- Plant Pathology Techniques: Proficient in disease diagnosis, pathogen isolation, inoculation methods, and molecular diagnostics (PCR, qPCR) Experienced with serological assays (ELISA), microscopy, and conducting field surveys for disease monitoring and epidemiological studies
- Molecular Biology Techniques: Proficient in QPCR, DNA extraction, plant disease diagnosis and inoculation, fungal culture, and protocol development Experienced in applying these techniques for pathogen identification, gene expression analysis, and disease management studies
- Bioinformatics $ Data Analysis: Skilled in genomic data analysis using R for biostatistics, GWAS analysis, QTL mapping, and data visualization Proficient in scripting and automation with Linux (Bash), Perl, Python, and Java for handling large datasets, variant calling, and de novo genome assembly
- Research Design: Experienced in designing and implementing experimental and observational studies, including hypothesis development, variable identification, sampling strategies, data collection methods, and statistical analysis for plant disease diagnostics and epidemiology studies
Awards
- Best Student Poster Presentation, CPS-SK and PSSA Sixth Joint Meeting, 2023
- Robert Travel Award, 2022
- PPSA Graduate Student Scholarship, 2021
- University of Alberta Doctoral Recruitment Scholarship, 2020
- Best Student Oral Presentation, 40th Plant Pathology Society of Alberta, 2019
- Best Student Oral Presentation, Graduate Student Symposium in Lethbridge Research and Development Center, 2019
- Major Scholarship of Northeast Forestry University, 2014-2016
Publications
- B. Wei, R. Gourlie, R. Celoy, E. Amundsen, and R. Aboukhaddour. Stripe rust virulence and host reaction assessment protocols: from lab to field, Wheat Rusts: Methods and Protocols, 2024
- R. Aboukhaddour and B. Wei, Foliar diseases of cereals in southern Alberta, 2023, Canadian Journal of Disease Survey, 2024
- M.W. Harding, T.K. Turkington, S. Rehman, S. Waterman, H. Klein-Gebbinck, R. Aboukhaddour, N. Rauhala, B. Wei, M. Zid, G.C. Daniels, R. Neeser-carazo and L. Stellar, Wheat foliar disease survey in Alberta, 2022, Canadian Journal of Disease Survey, 2023
- B. Wei, M. Zid, and R. Aboukhaddour, Stripe rust of cereals in Alberta, 2021, Canadian Journal of Disease Survey, 2022
- M.W. Harding, T.K. Turkington, S. Waterman, H. Klein-Gebbinck, R. Aboukhaddour, N. Rauhala, B. Wei, M. Zid, K. McCormack, G.C. Daniels, and M.A. Kennedy, Wheat Leaf Disease Survey in Alberta, 2021, Canadian Journal of Disease Survey, 2021
- B. Wei, T. Despins, M.R. Fernandez, S.E. Strelkov, Y. Ruan, R. Graf and R. Aboukhaddour, Race Distribution of Pyrenophora tritici-repentis in relation to ploidy level and susceptibility of durum and winter bread wheat, Canadian Journal of Plant Pathology, 2021
- B. Wei, M.J. Moscou, K. Sato, R. Gourlie, S.E. Strelkov, and R. Aboukhaddour, Identification of a locus conferring dominant susceptibility to Pyrenophora tritici-repentis in barley, Frontiers, in Plant Science, Plant Breeding, 2020
- B. Wei, K. Zhang, H. Tang, J. Shen, and R. Song, The insecticidal activities of ethyl acetate efficiently extracts from fifteen bio-control strains to PineWood nematodes, Forestry Science & Technology, 2017
Conferences
- Genomic analysis of stripe rust populations in Canada, CPS Annual Meeting, 07/07-10, 2024. Winnipeg, Manitoba, Canada
- Genetic analysis of Puccinia striiformis (stripe rust) in Canada, RDAR Research Showcase, 01/11, 2024. Edmonton, Alberta, Canada
- Genetic analysis of Puccinia striiformis (stripe rust) in Canada, Sixth Joint Meeting of CPS-SK and PPSA, 10/30-11/01, 2023. Lloydminster, Alberta, Canada
- Virulence of Puccinia striiformis (stripe rust) in Canada from 2020 to 2022, Canadian Tri-Society Meeting, 06/19, 06/21, Ottawa, Ontario, Canada
- Stripe rust in Alberta threatens the Canadian wheat, PPSA Annual Meeting, 11/04-06, 2022. Brooks, Alberta, Canada
- Novel mutations in the CYP51 gene detected in Pyrenophora tritici-repentis (tan spot of wheat), Tri-Society Virtual Conference, 07/05-09, 2021. Remote
- Identification of a dominant locus for susceptibility to tan spot in barley, Lethbridge Research and Development Centre Graduate Student Symposium, 11/21, 2019. Lethbridge, Alberta, Canada
- Identification of a dominant locus for susceptibility to tan spot in barley, PPSA Annual Meeting, 11/04, 11/06, Lacombe, Alberta, Canada
- Virulence of Pyrenophora tritici-repentis in relation to host and prevalence of Tsn1 gene in winter and durum wheat, PPSA Annual Meeting, 11/04-06, 2019. Lacombe, Alberta, Canada
- Virulence of Pyrenophora tritici-repentis in relation to host and prevalence of Tsn1 gene in winter and durum wheat, CPS Annual Meeting, 07/06-08, 2019. Guelph, Ontario, Canada
- The Ptr-barley interaction is specific and is controlled by a single locus, International Symposium on Cereal Leaf Blights, 05/22-24, 2019. Dublin, Ireland
Certification
- Certificate of Green Software for Practitioners The Linux Foundation 2024
- Certificate in Autism and Neurodiversity in Early Childhood Care UBC 2024
- Certificate of Rapid Teacher Training Programme on Distance, Open and Online Learning UNESCO 2024
- WHMIS University of Alberta 2020
- First Aid Certification 2015
Timeline
Research Affiliate
Agriculture and Agri-Food Canada, AAFC
12.2020 - Current
Research Participant
Agriculture and Agri-Food Canada, AAFC
05.2018 - 08.2020
- Certificate of Green Software for Practitioners The Linux Foundation 2024
- Certificate in Autism and Neurodiversity in Early Childhood Care UBC 2024
- Certificate of Rapid Teacher Training Programme on Distance, Open and Online Learning UNESCO 2024
- WHMIS University of Alberta 2020
- First Aid Certification 2015
Ph.D. - Plant Science
University of Alberta
Master of Science - Plant Science
University of Alberta
Bachelor of Science - Forestry
Northeast Forestry University
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