Summary
Overview
Work History
Education
Skills
Websites
Research
References:
Timeline
Generic

Pranav Sood (Permanent Resident Of Canada)

Toronto,Canada

Summary

Biotechnology (Microbiology, CAR-T, Upstream Manufacturing of Biosimilars, R & D in IVD kits) professional with 6 years of experience as a researcher. Extensive experience with cGMP,GLP, Aseptic laboratory techniques and equipment. Exceptional organizational and problem-solving skills.

Overview

7
7
years of professional experience

Work History

Research Associate

Institute for Life Sciences Entrepreneurship (ILSE)
, USA
04.2018 - 07.2019
  • Executed DNA extraction, quantification, sample incubation, and cryopreservation
  • Managed the archiving and upkeep of a diverse library of over 300 microbial strains, including bacteria, yeast, actinomycetes, and fungi
  • Generated and reported NanoDrop and Qubit findings; regularly conducted PCR, restriction digestion, and gel electrophoresis
  • Conducted MIC and MBC assays on 100+ strains to evaluate antibiotic effects, utilizing 96-well plate assays, and carried out synergy tests
  • Investigated spontaneous resistance in gram-positive bacteria to target antibiotics
  • Proficiently operated within an anaerobic chamber AS-580 environment
  • Cultivated and cryopreserved 250+ microbial strains post-log phase confirmation
  • Determined CFU using optical density measurements and dilution plating techniques
  • Executed chemical inactivation of bacteria, ensuring the preservation of cellular structure and DNA
  • Verified cellular integrity post-inactivation via cell imaging and flow cytometry
  • Authored SOPs and work instructions tailored to specific bacterial strains
  • Applied CRISPR/Cas9 technology for the targeted genetic editing of selected organisms
  • Performed a study on spontaneous resistance to identify how often and through what mechanisms gram-positive bacteria develop reduced susceptibility to a specific antibiotic

Sr. Research Associate

Institute for Life Sciences Entrepreneurship (ILSE)
, USA
08.2019 - 12.2019
  • Managed and executed multiple projects autonomously, utilizing advanced microbiological and molecular laboratory techniques
  • Contributed technical and scientific expertise to research leadership

CAR-T Scientist

Eurofins PSS (Client: Johnson & Johnson)
, USA
01.2020 - 08.2021
  • Conducted NK and Daudi Cell Line Maintenance
  • Sample Testing
  • Utilized Viral Copy number, vesicular stomatitis virus G protein (DNA Isolation and qPCR using quant studio 6) for detecting presence and amount of Lentiviral (Transforming agent) DNA in the patient CAR-T cells
  • NC200 cell counter for cell count and viability of the patient cells
  • Beckman Coulter Lyric flow cytometry (3C, 5C, 8C) for patient sample analysis of cell groups
  • Analyzed results and documented data in the Electronic Lab Notebook (ELN-BIOVIA)
  • Executed Tumor Killing Assays (using microplate based luminous assay machine-Spectra max L) to evaluate the efficacy of CAR-T drug products
  • Trained and supervised new lab personnel in standard procedures and techniques

Upstream Manufacturing Scientist

Unicon Pharma (Client: Kashiv BioSciences)
, USA
08.2022 - 05.2023
  • Oversaw the culture and expansion of CHO cell line, adhering to set protocols and production schedules
  • Maintained aseptic conditions and upheld quality control standards throughout the cell culture process
  • Optimized cell production for better yield, purity, and efficiency
  • Conducted quality control assessments during manufacturing to ensure compliance with regulatory standards
  • Updated and maintained SOPs, aligning with GMP regulations
  • Led the execution of manufacturing batches, managing bioreactors and other essential equipment operations
  • Addressed and resolved process deviations and equipment malfunctions to maintain production continuity
  • Maintained and increased CHO cells for production needs; review manufacturing records and SOPs
  • Entrusted with the overall accountability of administering the quality management systems, documenting the deviations, deploying the change controls, and generating the codes for new materials
  • Inoculated CHO cells and managed growth in 250mL and 500mL shake flasks; scaled processes to 50L and 500L bioreactors
  • Monitored cell growth using Vi-cell Metaflex, pH meters, osmometers, and Vi-cell XR analyzers
  • Conducted product harvesting and filtration with a 3M system; verified filter integrity
  • Prepared formulation buffers and sampled water for injection (WFI) for manufacturing use
  • Collaborated with R&D to scale benchtop processes to manufacturing levels, refining techniques for increased scalability
  • Coordinated with interdisciplinary teams to ensure consistency in achieving project goals and adherence to timelines
  • Explored and implemented new technologies to enhance manufacturing processes and product integrity.

R and D Scientist

Trivitron Healthcare (LabSystems Diagnostix in Europe)
, INDIA
02.2024 - 10.2024
  • Enzyme production and Oligo synthesis for R and D division under HOD guidance (situated on Finland)
  • Spearheaded the establishment of enzyme and oligo synthesis laboratories to support the development of DNA test kits for European markets
  • Setting up the R and D labs (Enzyme (using DH5-a E.coli) and Oligo synthesis labs) for a new division for IVD kits( to be sold in European markets)
  • Leading the projects for R and D in developing DNA test kits and optimization (Probes/Primers/Enzymes)
  • Structuring of the lab and other systems like SOPs, documentations
  • Production of Reverse Transcriptase and DNA polymerase using Biorad NGC and transformed DH5-a E.coli (Protein fractionation process)
  • Comparing the In-house synthesized enzymes with market giants
  • Execute oligo and Enzyme research in support of research goals, plans and supervisor input
  • Produce Oligos (Primers and Probes with different dyes), QC using Agilent Infinity 2 HPLC and Enzymes
  • Evaluate performance of the synthetic oligos and enzymes, QC using qPCR on Quant studio 5, Optimization
  • Engage in collaboration and interaction with team and partners to facilitate the development, characterization, and testing for project advancement
  • Lab Systems Diagnostics in Finland

Education

Master of Science - Biotechnology

Harrisburg University
05-2023

Masters - Biotechnology

Kean University
New Jersey, USA
05-2018

Skills

  • SOP Development
  • Lab Management
  • Microbiology & CAR-T
  • Research Management
  • Project Management
  • GMP & Aseptic Laboratory Techniques & Equipment
  • Quality Assurance
  • Cell Line Maintenance
  • Client Engagement
  • Team Management
  • GLP
  • GMP
  • Upstream processing
  • Excellent Aseptic Techniques
  • Making the Cells Competent
  • Cell Culture
  • MS Office
  • ELN
  • Write SOPs under the project head's guidance
  • Extensive work in BSL-2 Lab
  • Anaerobic chamber (AS-580)
  • Work on Cell imager (Quantom-M50)
  • Cell flow cytometer (Beckman Coulter and BD Lyric)
  • Developed protocol for bacterial transformations
  • Interpret and summarize experimental data
  • Maintained Bacterial cell cultures
  • Plasmid DNA cloning
  • Plasmid Purification
  • Performed ELISA
  • RT-PCR (Quant Studio 6)
  • Autoclave and waste disposal
  • Documenting the results (ELN-Biovia)
  • Maintaining laboratory equipment
  • Maintaining cancer cell lines
  • Laboratory maintenance
  • Inventory
  • Purchasing
  • Reagent preparation
  • Editing Bacterial Genome (CSIPR/CAS)
  • Strain archiving
  • DNA extraction
  • Spectrophotometers
  • Preparation of LB, MS, CRA, TKB, TSC and various other mediums
  • Genomic DNA isolation from Bacterial cells
  • Invasion Assays
  • Cell line maintenance
  • Autoclave operations
  • MerMade 192E Oligo synthesizer
  • HPLC/MS
  • K and H Oligo synthesizer
  • BioRad NGC chromatograph
  • Quant studio 5/7
  • Maintaining laboratory records
  • Sonication
  • SDS-PAGE
  • Western Blot
  • ELISA
  • RT-PCR
  • Techniques involving Induction and cloning of cell

Research

  • Kean University, New Jersey, New Jersey, USA, (01/01/18 to 06/30/18), Transformation of Bacillus strain using CRISPR/Cas genome editing technology and development of an in-house electroporation protocol., The project involved genetic manipulation/transformation of Bacillus subtilis and E.coli using CRISPR/Cas9. Plasmid extraction using a Mini-prep kit. Plasmid with the target genes was introduced to the target organism using electroporation and then cloned. Designed protocols for bacterial transformations.
  • ATCC-CTM, New Jersey, New Jersey, USA,(05/01/17 to 09/30/17), Stimulation of Shigella Flexineri surface in the presence of bile salts at different concentrations., The project involved studying the stimulation of Shigella Flexineri in the presence of different bile salts at different concentrations on MDCK cells. It involved cell passaging, Assay plating and thawing. Invasion assays were also performed.

References:

ILSE Vice President - Dr. Robert McLaughlin, rmclaughlin@ilsebio.com

Eurofins Manager- Benjamin Bramble, email ID- BenjaminBramble@eurofinsus.com

Kashiv Bio Manager- Thomas Bednarz, email ID- thomasbednarz@yahoo.com

Trivitron/Lab SystemsDx Head of Deparment- Dr. Kevin Eboigbodin, email ID kevin.eboigbodin@labsystemsdx.com

Timeline

R and D Scientist

Trivitron Healthcare (LabSystems Diagnostix in Europe)
02.2024 - 10.2024

Upstream Manufacturing Scientist

Unicon Pharma (Client: Kashiv BioSciences)
08.2022 - 05.2023

CAR-T Scientist

Eurofins PSS (Client: Johnson & Johnson)
01.2020 - 08.2021

Sr. Research Associate

Institute for Life Sciences Entrepreneurship (ILSE)
08.2019 - 12.2019

Research Associate

Institute for Life Sciences Entrepreneurship (ILSE)
04.2018 - 07.2019

Master of Science - Biotechnology

Harrisburg University

Masters - Biotechnology

Kean University

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Pranav Sood (Permanent Resident Of Canada)