Experienced biomedical researcher and licensed pharmacist with a strong background in pharmacology, biotechnology, and molecular biology. PhD in Medical Science with postdoctoral-level research experience on insulin granule fractionation and β-cell function. Proven ability in designing and executing complex cell-based assays, immunostaining, radiolabeling techniques, and data analysis. Published first-author paper in Diabetes journal on SNAT5-related incretin signaling pathways. Fluent in Uyghur, Chinese (Mandarin), Japanese, and English, with cross-cultural academic and industry experience in China, Japan, and Canada. Passionate about contributing to research that bridges laboratory insights with clinical relevance.
• Performed in vitro differentiation of HL-60 promyelocytic cells into neutrophil-like cells for immunological assays.
• Conducted immunostaining using specific monoclonal antibodies to confirm cellular differentiation stages.
• Analyzed cell surface marker expression and morphological changes using fluorescence microscopy and image analysis.
• Collaborated with senior researchers and contributed to research reports and internal presentations.
• Maintained sterile cell culture environments and optimized differentiation protocols for consistent results.
• Conducted subcellular fractionation of insulin granules from MIN6 pancreatic β-cells using Nycodenz and Percoll gradient centrifugation.
• Prepared and optimized single-molecule tracking (SMT) buffers and other reagents for β-cell homogenization.
• Performed insulin secretion studies using radiolabeled glucose tracers (e.g., ¹⁴C-glucose, ³H-glucose) and Western blotting.
• Maintained detailed experimental records and collaborated in lab meetings to support ongoing β-cell physiology projects.
• Acquired practical skills in molecular biology, radioactive labeling, and diabetes-related cellular research.
• Dispensed and managed medications for hospitalized patients in the inpatient pharmacy.
• Collaborated with physicians and nurses to ensure accurate medication delivery and dosage monitoring.
• Gained practical experience in hospital pharmacy operations, patient safety, and medication management in a multidisciplinary setting.
Laboratory techniques
Research planning
Results interpretation
Data analytics
Image analysis
Research and publication
Electron microscopes
Experimental design
Experiment protocol development
Microsoft Excel proficiency
Effective multitasking
Laboratory studies
Conference Presentations & Participation
• 2011 – Presented at the 26th Annual Meeting of the Japanese Society for the Study of Xenobiotics (JSSX), Hiroshima, Japan
• 2015 – Presented PhD research at the 58th Annual Meeting of the Japanese Diabetes Society Conference, Shimonoseki, Japan
• 2016 – Gave a poster presentation at the annual meeting of the European Association for the Study of Diabetes, EASD 2016. Munich, Germany
• 2017 – Presented at the Incretin Research Meeting, kobe, Japan
• 2018 – Presented PhD research at the 61th Annual Meeting of the Japanese Diabetes Society Conference, Tokyo, Japan
Publication:
"Inhibition of SNAT5 Induces Incretin-Responsive State From Incretin-Unresponsive State in Pancreatic B-Cells: Study of B-Cell Spheroid Clusters as a Model"
Authors: Mahira Hashim, Norihide Yokoi, Harumi Takahashi, Ghupurjan Gheni, Oduori S. Okechi, Tomohide Hayami, Naoya Murao, Shihomi Hidaka, Kohtaro Minami, Akira Mizoguchi, Susumu Seino
Published in: Diabetes, 2018; 67(9): 1795-1806
DOI: 10.2337/db17-1486
PubMed: 29954738
"Please note that my name on this publication appears as Mahira Hashim, which was my name prior to becoming a Japanese citizen. After my graduation, my family and I adopted a new family name in accordance with Japanese regulations. My current name is Mahira Muhamadeari."
International Research Journey
With academic and professional experience in China, Japan, and Canada, I have developed a flexible and adaptive mindset toward research and collaboration. Exposure to diverse research environments and interdisciplinary teams has shaped my ability to contribute across cultures and integrate varied scientific approaches. These experiences continue to fuel my commitment to translational science that bridges laboratory discoveries and clinical needs.