Mona Abdel Hameed

I have had the opportunity to engage with various departments within the company. Initially, I joined the IVD group where I addressed issues encountered during protein purifications. Subsequently, I transitioned to the protein purification group, where I pioneered a groundbreaking protocol for extracting Myelin Basic Protein (MBP) from pig brain without the use of chloroform. This innovation not only optimized efficiency by reducing extraction time from 7 days to one, but also significantly minimized associated hazards, particularly the use of chloroform. Presently, I am involved in the plant department, where my focus lies in researching proteins that pose challenges in bacterial expression, utilizing plants as an alternative expression system.

Work procedures involve: -
 Conducting protein purifications across diverse expression systems.
 Developed an innovative protocol for the extraction of Myelin Basic Protein (MBP) from pig brain without the use of chloroform, resulting in superior yield and quality.
 Utilizing plant expression systems for the synthesis of various proteins.
 Incorporating noncanonical amino acids into different proteins through the utilization of diverse orthogonal systems. This project involves site-specific phosphorylation of various proteins, including kinases and tau proteins.
 Engaged in the design of different linkers for Antibody Drug Conjugates (ADCs) using site-specific incorporation systems.

• I have had the opportunity to engage with various departments within the company
• Initially, I joined the IVD group where I addressed issues encountered during protein purifications
• Subsequently, I transitioned to the protein purification group, where I pioneered a groundbreaking protocol for extracting Myelin Basic Protein (MBP) from pig brain without the use of chloroform
• This innovation not only optimized efficiency by reducing extraction time from 7 days to one, but also significantly minimized associated hazards, particularly the use of chloroform
• Presently, I am involved in the plant department, where my focus lies in researching proteins that pose challenges in bacterial expression, utilizing plants as an alternative expression system
• Work procedures involve: - Conducting protein purifications across diverse expression systems
• Developed an innovative protocol for the extraction of Myelin Basic Protein (MBP) from pig brain without the use of chloroform, resulting in superior yield and quality
• Mona Abdel-Hameed, BSc, MSc, PhD, Ph
• 204 509 4950 Email mona.abdelshafi@gmail.co Page 3 Utilizing plant expression systems for the synthesis of various proteins
• Incorporating noncanonical amino acids into different proteins through the utilization of diverse orthogonal systems
• This project involves site-specific phosphorylation of various proteins, including kinases and tau proteins
• Engaged in the design of different linkers for Antibody Drug Conjugates (ADCs) using site-specific incorporation systems
• In this role, my primary objective was to establish a new biochemistry laboratory for a faculty member in the Department of
• Chemistry
• Collaborating with Dr
• Budisa, a prominent figure in genetic code expansion, we embarked on a groundbreaking experiment involving the evolution of bacteria and the alteration of its genetic code
• Additionally, I conducted experiments involving the incorporation of non-canonical amino acids into various proteins using different auxotrophic bacterial strains
• Some of the key responsibilities and accomplishments during this tenure include: - Spearheading the establishment of the new laboratory and procuring all necessary instruments and equipment for biochemistry research
• Conducting and providing support for scientific investigations and experiments, including planning, setup, and execution of controlled trials
• Recording, analyzing, and interpreting experimental data to draw meaningful conclusions
• Demonstrating experimental procedures and ensuring adherence to scientific protocols
• Collecting, preparing, and testing samples with meticulous attention to detail
• Maintaining, calibrating, and ensuring the sterility of laboratory equipment to uphold research standards
• Offering technical support to fellow researchers and presenting results to senior staff members
• Drafting comprehensive reports, reviews, and summaries of research findings
• Staying abreast of relevant scientific and technical developments to inform research practices
• Writing reports, reviews and summaries Supervising laboratory staff and overseeing stock inventory and resource management
• Furthermore, I managed several research endeavors, including evolution experiments focused on substituting essential amino acids with synthetic counterparts to observe bacterial tolerance to the alterations
• Moreover, I conducted in vivo incorporations of synthetic, non-canonical amino acid analogs in CSPB, examining the resultant protein's activity and structure post-incorporation.

• Kontzamanis graumann smith macmillan inc
• Project: Large-Scale Cultivation of Two Strains of KGS Bacteria
• The primary objective of this project was to explore the biosynthetic potential of two strains of KGS bacteria and assess the bioactivity of their metabolites against fungi known to infect crops
• Key accomplishments and methodologies employed during the project include: Optimization of Growing Conditions: Investigated and determined the optimal growth parameters for cultivating KGS
• Mona Abdel-Hameed, BSc, MSc, PhD, Ph
• 204 509 4950 Email mona.abdelshafi@gmail.co Page 4 bacteria to facilitate the production of secondary metabolites on a large scale
• Organic Solvent Extraction: Conducted organic solvent extraction of various batches of KGS 3 fermentation media to isolate metabolites
• Analyzed the efficacy of each extraction process to maximize yield and purity
• Chromatographic Analysis: Utilized Thin Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) to analyze crude extracts obtained from KGS 3 fermentation
• This analytical approach enabled the identification and quantification of metabolites present in the extracts
• Optimization of Chromatographic Isolation: Developed and optimized conditions for Accelerated Chromatographic
• Isolation (ACI) to fractionate the crude extract of KGS 3 into distinct 'fractions' based on polarity
• This facilitated the isolation of specific metabolites for further analysis
• Chemical Characterization: Employed a combination of TLC, HPLC, and Nuclear Magnetic Resonance (NMR) spectroscopy to chemically analyze each fraction obtained through ACI
• This comprehensive approach allowed for the identification and structural elucidation of individual metabolites present in the crude extract
• Interpretation of Results: Analyzed and interpreted the results obtained from TLC, HPLC, and NMR analyses to elucidate the chemical composition and structural characteristics of the isolated metabolites
• This critical step provided insights into the bioactivity and potential applications of the identified compounds, including their antifungal properties.

• At this company, our primary focus centered around phages with the objective of developing a novel phage capable of targeting multiple bacterial strains simultaneously
• The core responsibilities encompassed isolating, purifying, and cultivating phages sourced from diverse environmental samples
• Additionally, extensive molecular biology techniques and analytical chemistry methodologies were employed to characterize the isolated phages
• Some of the key roles undertaken during my tenure included: Bacteriophage Extraction and Analysis: Led the extraction, analysis, and large-scale production of bacteriophages derived from varied environmental sources
• This involved meticulous purification processes to obtain highly concentrated phage solutions for subsequent experimentation
• Molecular Biology Techniques: Applied a comprehensive array of molecular biology techniques, including PCR amplification, gel electrophoresis, DNA sequencing, and de novo genome assembly, to elucidate the genetic composition and structure of phage genomes
• This enabled a detailed examination of phage diversity and genomic organization
• Annotation of Secondary Metabolite Gene Clusters: Utilized advanced bioinformatics tools such as Antismash software and Blast searches to annotate secondary metabolite gene clusters within phage genomes
• This facilitated the identification of potential bioactive compounds and contributed to the understanding of phage-mediated interactions with bacterial hosts
• Protein Expression and Structural Analysis: Conducted protein expression studies and performed comprehensive structural analyses to elucidate the functional properties and virulence determinants of phage-encoded proteins
• This involved protein modeling and in-depth analysis of protein-protein interactions to delineate their roles in phage-host interactions
• Literature Reviews: Conducted extensive literature reviews focusing on emerging research targets within diverse ecosystems, including swine, cattle, and fish
• This provided valuable insights into the evolving dynamics of phage- bacteria interactions and informed future research directions.

• Demonstrated exceptional leadership qualities while managing multiple projects simultaneously under tight deadlines without sacrificing quality or accuracy.
• Facilitated cross-departmental communication, ensuring seamless integration of resources for successful project completion.
• Collaborated with [Type] professionals to advance research and gain deeper understanding of topics.
• Maintained high levels of confidentiality to protect data quality and project research.
• Contributed to planning appropriate and engaging lessons for both classroom and distance learning applications.
• Edited papers for submission to prestigious academic and industry journals.
• Evaluated and supervised student activities and performance levels to provide reports on academic progress.
• Bolstered cybersecurity measures by deploying state-of-the-art software solutions and regularly updating employee training protocols.
• Developed high-performing teams by fostering a positive work environment, emphasizing collaboration, and setting clear expectations.
• Contributed to research activities by providing specialist input based on professional expertise, practice, experience, and qualifications.
• Transformed company culture by promoting diversity, equity, inclusion principles throughout all organizational levels leading to increased morale among staff members.
• Published research in high impact journals.
• Optimized workflow efficiency by streamlining processes and eliminating unnecessary steps in project management.
• Proven ability to develop and implement creative solutions to complex problems.
• Acted as a team leader in group projects, delegating tasks and providing feedback.
• Assisted with day-to-day operations, working efficiently and productively with all team members.
• Gained strong leadership skills by managing projects from start to finish.
• Organized and detail-oriented with a strong work ethic.
• Used critical thinking to break down problems, evaluate solutions and make decisions.
• Demonstrated respect, friendliness and willingness to help wherever needed.
• Used strong analytical and problem-solving skills to develop effective solutions for challenging situations.
• Worked well in a team setting, providing support and guidance.
• Managed time efficiently in order to complete all tasks within deadlines.
• Learned and adapted quickly to new technology and software applications.
• Developed strong communication and organizational skills through working on group projects.
• Cultivated interpersonal skills by building positive relationships with others.
• Identified issues, analyzed information and provided solutions to problems.
• Adaptable and proficient in learning new concepts quickly and efficiently.
• Excellent communication skills, both verbal and written.
• Provided professional services and support in a dynamic work environment.
• Resolved problems, improved operations and provided exceptional service.
• Demonstrated leadership skills in managing projects from concept to completion.
• Proved successful working within tight deadlines and a fast-paced environment.
• Assisted with collecting, identifying and packaging hazardous and non-hazardous waste products to comply with Resource Conservation and Recovery Act regulations.
• Maintained organized, safe and efficient laboratory environment to minimize accidents and prevent cross contamination.
• Contributed to team discussions and new project initiatives to advance progress and optimize profitability.
• Reduced production costs through the design and synthesis of novel, cost-effective materials.
• Leveraged computerized management system and labeling process to conduct inventory of reagents, chemicals and supplies.
• Implemented new analytical techniques, enhancing accuracy and speed of data collection for critical decision-making processes.
• Collaborated with cross-site and cross-functional teams in research and development, EH&S, product marketing and manufacturing engineering to establish framework for successful development programs and align objectives across teams.
• Streamlined testing procedures, ensuring accurate results while minimizing turnaround time.
• Enhanced product quality by developing and optimizing chemical processes and procedures.
• Provided technical support to production team, troubleshooting issues and ensuring a seamless transition from lab scale to full-scale manufacturing.

• Project: Large-Scale Production of Astaxanthin from Microalgae Haematococcus pluvialis
• The project aimed to achieve the large-scale production of astaxanthin from microalgae Haematococcus pluvialis through a series of systematic procedures: Optimization of Growing Conditions: Conducted comprehensive studies to determine the optimal growing conditions for microalgae Haematococcus pluvialis, focusing on maximizing biomass production of astaxanthin
• Various growth media and stress-inducing factors were systematically evaluated to enhance astaxanthin yield
• Analytical Chemistry Assays: Utilized advanced analytical instrumentation, including HPLC (High-Performance Liquid
• Chromatography) and NMR (Nuclear Magnetic Resonance) techniques, to perform detailed analytical chemistry assays
• These assays facilitated the accurate quantification and characterization of astaxanthin and other carotenoids present in the microalgae extracts
• Development and Validation of Analytical Testing Methodology: Established and validated analytical testing methodologies to assess the efficacy of different conditions for astaxanthin production
• This involved rigorous protocol development and validation procedures to ensure the reliability and reproducibility of experimental results
• Carotenoid Identification and Separation: Implemented a novel protocol for the identification and separation of various carotenoids present in microalgae extracts using HPLC
• This enabled precise characterization and isolation of astaxanthin and other carotenoid compounds, contributing to the optimization of extraction processes
• Characterization of Astaxanthin: Investigated the physical and chemical characteristics of astaxanthin under different environmental conditions using HPLC and NMR techniques
• This comprehensive characterization provided insights into the stability and functionality of astaxanthin in diverse settings
• Cultivation Expertise: Acquired extensive expertise in cultivating microalgae, yeast, and fungi under varied culture conditions, contributing to a deeper understanding of microbial physiology and growth dynamics
• Protocol Development for Extraction: Developed innovative extraction protocols to maximize the yield of astaxanthin and other valuable carotenoids from microalgae biomass
• These protocols were designed to be efficient, cost-effective, and environmentally sustainable.

• Project: Polyketide Biosynthesis in Lichen Fungi Cladonia Uncialis
• The project focused on elucidating polyketide biosynthesis pathways in the lichen fungi Cladonia uncialis, aiming to identify key gene clusters associated with usnic acid production and other secondary metabolites
• Key aspects of the project included: Multidisciplinary Approach: Applied a diverse array of molecular biology and analytical chemistry techniques, including
• PCR, Rapid Amplification of cDNA Ends (RACE), gel electrophoresis, DNA sequencing, de novo genome assembly and analysis, secondary metabolite gene cluster annotation using Antismash software, Blast search, protein expression, protein modeling, protein analysis, HPLC, and TLC
• Gene Cluster Identification: Successfully identified the usnic acid gene cluster through comprehensive de novo sequencing of the Cladonia uncialis genome and in silico analysis of polyketide biosynthetic gene clusters
• This pioneering effort shed light on the genetic basis of usnic acid biosynthesis in lichen fungi
• Discovery of Biosynthetic Gene Clusters: Identified a total of 56 putative biosynthetic gene clusters within the Cladonia uncialis mycobiont genome, encompassing diverse enzyme classes such as non-ribosomal peptide synthetases (NRPSs), polyketide synthases (Type I highly reducing, partially reducing, and non-reducing synthases, as well as Type III PKSs), lanthipeptide synthases, terpene synthases, and hybrid genes.