Lu Ji
Union City
Summary
Postdoctoral fellow at Stanford Medicine specializing in oncology and immunology. Advanced radiation therapy efficacy through innovative FTO inhibition strategies and developed therapeutic targets for gastric cancer immunotherapy. Proven leadership in managing research projects and collaborations, resulting in multiple peer-reviewed publications. Strong communicator with expertise in experimental design and execution.
Overview
9
9
years of professional experience
Work History
Postdoctoral Fellow
Stanford Medicine
02.2022 - Current
Project: Exploring the potential of FTO inhibition to enhance radiation therapy efficacy in HNSCC.
- Demonstrated anti-tumor effects of radiation combined with FTO inhibition (genetic and pharmacological) in syngeneic HNSCC mouse models.
- Uncovered cell-intrinsic therapeutic mechanisms in HNSCC using bulk RNA-seq, immunofluorescence, and homologous recombination repair assays.
- Profiled immune cells in the TME and uncovered cellular heterogeneity in HNSCC tumors using multiplex flow cytometry and single-cell RNA sequencing.
- Developed customized protocols tailored to specific research questions, ensuring optimal outcomes across various projects.
- Authored professional scientific papers for publishing in peer-reviewed journals.
- Collaborated across teams to drive research progress and finalize paper revisions.
PhD Graduate Student
Shanghai Jiao Tong University
08.2016 - 08.2021
Main Project: Exploring the potential immunotherapeutic targets for gastric cancer.
- Developed a spontaneous gastric cancer mouse model and validated tumor progression.
- Uncovered immune modulation in gastric cancer mice treated with CCL28/CD24/PD-1 antibody through immune cell population analysis.
- Uncovered molecular mechanisms by Investigating β-catenin-mediated transcriptional regulation of CCL28 .
- Revealed key immune interactions between Treg cells and gastric cancer cells in vitro.
- Engaged in multiple collaborations to publish scientific papers.
Education
Ph.D. - Biology, Immunology
Shanghai Jiao Tong University
Shanghai, China08-2021
Bachelor of Science - Bioengineering
East China University of Science And Technology
Shanghai, China07-2016
Skills
- Cell biology: extensive cell culture experience; generation of modified cell lines with shRNAs/siRNAs or cDNAs using viral systems (lentivirus and retrovirus) or chemical/liposome transfection; generation of primary cell lines from tissues (mesenchymal stem cells)
- Multiplex flow cytometry: skilled in processing tumor tissue, spleen, lymph nodes, and peripheral blood from tumor mouse models; proficient in profiling primary immune cells
- Cell based assays: clonogenic assay; MTS assay; cell cycle/apoptosis assay; transwell assay
- Molecular biology: isolation and analysis of DNA and RNA from cells/tissues; library preparation for scRNA-seq; PCR; qPCR; luciferase assay; ChIP assay
- Biochemistry: western blot; immunohistochemistry; immunocytochemistry
- In vivo techniques: use, handling and breeding of mouse models; establish subcutaneous tumor models; administration of compounds via ip injection and oral gavage
- IT tools: MS Office, Adobe suite, GraphPad, Flowjo, Image J
- Leadership and Management: planned, led and managed my own research projects; coached summer interns; developed experiments trouble-shooting skills
- Independence: full responsibility to lead my research projects; designed the projects and wrote the scientific papers
- Teamwork: Successful internal and external collaborations led to several publications, both during my PhD and the postdoc; taught/learnt new techniques to/from colleagues and collaborators
- Communication: Delivered poster presentations at international conferences; wrote scientific publications and PhD thesis
- Attention to detail: accurate record keeping of experiments; complete work in a timely manner; complete peer reviewed scientific publications
Publications
Ji, L., Pu, L., . . .Rankin, E.B. FTO inhibition enhances the therapeutic index of radiation therapy in head and neck cancer, JCI Insight. Accepted.
Ji, L., Peng, P., Gui, L., Yuan, P., Qian, W., Wang, Y., . . . Ma, B. (2022). CD24 Is a Superior Immunotherapeutic Target to PD-1 in a Mouse Model of Helicobacter-Induced Gastric Cancer. Gastro Hep Adv, 1(1), 79-82. doi:10.1016/j.gastha.2021.09.011
Ji, L., Qian, W., Gui, L., Ji, Z., Yin, P., Lin, G. N., . . . Gao, W. Q. (2020). Blockade of β-Catenin-Induced CCL28 Suppresses Gastric Cancer Progression via Inhibition of Treg Cell Infiltration. Cancer Res, 80(10), 2004-2016. doi:10.1158/0008-5472.CAN-19-3074
Wang, C., Yan, J., Yin, P., Gui, L., Ji, L., Ma, B., & Gao, W. Q. (2020). β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer. Oncoimmunology, 9(1), 1809947. doi:10.1080/2162402X.2020.1809947
Yin, P., Gui, L., Wang, C., Yan, J., Liu, M., Ji, L., . . . Gao, W. Q. (2020). Targeted Delivery of CXCL9 and OX40L by Mesenchymal Stem Cells Elicits Potent Antitumor Immunity. Mol Ther, 28(12), 2553-2563. doi:10.1016/j.ymthe.2020.08.005
Timeline
Postdoctoral Fellow
Stanford Medicine
02.2022 - Current
PhD Graduate Student
Shanghai Jiao Tong University
08.2016 - 08.2021
Ph.D. - Biology, Immunology
Shanghai Jiao Tong University
Bachelor of Science - Bioengineering
East China University of Science And Technology
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