Summary
Overview
Work History
Education
Skills
Timeline
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Jisha Jayakrishnan

Tracy,CA

Summary

Research Associate specializes in establishing project objectives, developing experimental plan and effectively prioritizing tasks to meet time sensitive delivery goals. Serves as point person for executing research projects and disseminating findings to leadership. Displays strong written and oral communication skills to present complex analyses effectively. Knowledge about mRNA vaccine process and development. Strong proficiency in cell-based assays and analytical techniques like ELISA, HCP,Pro A (ELISA), HPLC, CGE, CE, PCR, Gel electrophoresis,Western Blot, Agarose gel analysis,DNA Purification through Megmax. Ability to work with minimal supervision and be proficient in Microsoft Office, Excel, PowerPoint, and Word.

Overview

5
5
years of professional experience

Work History

Senior Research Associate

Gritstone Bio -(Pharmalogic Recruiting)
12.2022 - 06.2023
  • Support bio-assay method development, qualification, and transfer to quality control
  • Perform analytical procedures in support of characterization, stability, and testing of drug substances and products
  • Hands-on work experience in different types of assays like CE analysis (Purity analysis of RNA and DNA), trained on LC-MS, Zeta potential determination, and Particle Size Determination (DLS).
  • Ensure high-quality and timely documentation in established laboratory notebook.
  • Draft characterization analytical reports and certificates of analysis
  • Analyze or summarize experimental data and present it at group meetings.
  • Provide documentations and reports of all tests in timely on ELN with high quality requested standards.
  • Perform other duties as assigned, such as lab maintenance, ordering, and stock lab supplies
  • Gathered and organized information for research purposes

Scientist

Eurofins PSS-(Boehringer-Ingelheim)
03.2021 - 12.2022
  • Proficiency and experience in running analytical assays and bioassays
  • Analyses of in-process samples using analytical techniques Cation exchange chromatography (CEX) Capillary Electrophoreses (CE) and immunobased assays for impurity analysis (ELISA) and qPCR
  • Determining concentration of residual proteins such as CHOP and residual protein A by ELISA (Cygnus assay), residual DNA by automated DNA extraction and real-time PCR
  • Documented all processes during experiments, keeping meticulous notes of unexpected changes
  • Conducted experiments for product development purposes in laboratory settings
  • Collaboratively and efficiently work with group to deliver targets within deadlines
  • Experience in working with real-time PCR systems and MegMAX automated DNA extraction systems
  • Troubleshoot experimental roadblocks and discuss problems with team members to arrive at potential solutions
  • Performed data analysis, reviewing and storing ELN notebooks based on company policies
  • Ability to work independently, efficiently, and productively in a fast-paced, highly collaborative, and diverse team environment.

RESEARCH ASSOCIATE

Neobiotechnology
02.2018 - 02.2021
  • Hands-on-experience with Immunoassays, / IHC (immunohistochemistry), Westernblot, and SDS-PAGE
  • Routinely performed immuno-histological staining and procedures for screening of monoclonal antibodies
  • Produces average of 300 IHC slides per week to screen for and validate positives during monoclonal antibody development at various stages while managing multiple projects with overlapping deadlines
  • Assisted in daily laboratory operations but not limited to maintaining reagent levels, making up of antibodies, dilutions, and daily maintenance of lab equipment
  • Perform and assist other assays for characterization of antibodies using different techniques like SDS PAGE and
  • Western blot
  • Proven ability to organize, prioritize and assist with multiple research projects
  • Design and develop immunoassays for known and novel targets, optimize SOPs for new formulations (e.g
  • Cocktails, multiplex, pre-diluted ready-to-use format); Participate in validation of antibodies for use in immune assay
  • Performed receiving, manufacturing, aliquoting and storing of Monoclonal Antibodies
  • Trains new staff and interns in laboratory techniques as well as lab safety and equipment maintenance, as well as documentation procedures
  • Assumes responsibility for keeping work area clean, neat and organized
  • Completes thorough cleaning at end of work period.

Education

Master of Science - Biotechnology

Madurai Kamaraj University

Bachelor of Science - Microbiology

Periyar University

Certificate of Achievement in Biotech Bio-manufacturing -

Ohlone College
Newark, CA

Certificate of Achievement in Biotech Research -

Ohlone College
Newark, CA

Skills

  • Basic Laboratory skills
  • Pipetting and making solutions
  • Preparing media and reagents, serial dilution
  • Aseptic Culture techniques
  • Microbiology: Aseptic techniques
  • Culture on agar plates, collecting microorganisms from air and water, Isolation and identification of microorganisms
  • Sample preparation,pH measurement
  • Gel electrophoresis, Agarose gel
  • HPLC,CEX-Cation Exchange Chromatography
  • ELISA (ELISA plate reader with SoftMax Pro)
  • CHOP ELISA, Pro A ELISA
  • PCR, qPCR
  • DNA and Plasmid DNA Purification
  • RNA sample prep and analysis
  • SDS-PAGE, Western Blot
  • Immunohistochemistry
  • Gel Analysis
  • Mammalian cell Culture: cell seeding, cell passage
  • Data Analysis
  • Particle size distribution (DLS)

Timeline

Senior Research Associate

Gritstone Bio -(Pharmalogic Recruiting)
12.2022 - 06.2023

Scientist

Eurofins PSS-(Boehringer-Ingelheim)
03.2021 - 12.2022

RESEARCH ASSOCIATE

Neobiotechnology
02.2018 - 02.2021

Master of Science - Biotechnology

Madurai Kamaraj University

Bachelor of Science - Microbiology

Periyar University

Certificate of Achievement in Biotech Bio-manufacturing -

Ohlone College

Certificate of Achievement in Biotech Research -

Ohlone College

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Jisha Jayakrishnan