SHRIKESH SACHDEV
Unionville,ON
Overview
1
1
Language
35
35
years of professional experience
Work History
Senior Research Associate
University of Missouri
10.2023 - 03.2026
- Mentor: Dr. Gary A. Weisman, Curators’ Distinguished Professor Biochemistry
- Biochemistry; Curators’ Distinguished Professor; Professor of Plant Science and Technology; Assistant Professor of Veterinary Pathobiology and Director of Molecular Interactions Core; Curators’ Professor and Biochemistry Member, National Academy of Sciences; Research Professor, Animal Sciences; Comparative Oncology, Radiobiology, and Epigenetics Laboratory; Department of Medicine, Cardiology; Gene Center and Institute of Biochemistry; Bond Life Sciences Center; University of Munich; Harry S. Truman Memorial Veterans Hospital
- Designed and conducted complex research studies to advance scientific knowledge in relevant fields.
- Collaborated with interdisciplinary teams to develop innovative methodologies and improve research outcomes.
- Analyzed data using statistical software to ensure accuracy and reliability of research findings.
Senior Research Associate
University of Missouri
09.2023 - 09.2023
- Guided students in plant science under Dr. David Mendoza-Cózatl
- Taught courses in plant science and technology at the university level
Senior Research Associate
University of Missouri
07.2022 - 08.2023
- Mentor: Dr. Kamendra Singh, Assistant Professor of Veterinary Pathobiology and Director of Molecular Interactions Core
- Assistant Professor of Veterinary Pathobiology and Director of Molecular Interactions Core
Senior Research Associate
University of Missouri
08.2019 - 06.2022
- Mentors: Dr. R. Michael Roberts, Curators’ Professor and Biochemistry Member, National Academy of Sciences and Dr. Toshi Ezashi, Research Professor, Animal Sciences
- Curators’ Professor and Biochemistry Member, National Academy of Sciences; Research Professor, Animal Sciences
Senior Research Associate
University of Missouri
09.2018 - 07.2019
- Mentor: Dr. Angela McCleary-Wheeler
- Comparative Oncology, Radiobiology, and Epigenetics Laboratory
Senior Research Scientist
University of Missouri
07.2018 - 08.2018
- Mentor: Dr. Chandrasekar Bysani, Margaret Proctor Mulligan Endowed Professor Research Career Scientist-Harry S. Truman Memorial Veterans Hospital Department of Medicine-Cardiology. University of Missouri
- Department of Medicine-Cardiology
Senior Research Scientist
University of Missouri
08.2011 - 07.2018
- Mentor: Dr. David J. Pintel, Curators Distinguished Professor Dr. R. Philip and Diane Acuff Endowed Professor in Medical Research Molecular Microbiology and Immunology University of Missouri.
Research Scientist
University of Missouri
08.2009 - 07.2011
- Mentor: Dr. Mark Hannink, Professor of Biochemistry Associate Director, Fellowships and Education, Bond Life Sciences Center University of Missouri.
- Associate Director, Fellowships and Education, Bond Life Sciences Center
Postdoctoral Fellow
University of Missouri
04.2003 - 08.2009
- Mentor: Dr. Mark Hannink, Professor of Biochemistry Associate Director, Fellowships and Education, Bond Life Sciences Center University of Missouri.
- Associate Director, Fellowships and Education, Bond Life Sciences Center
Fellow of the American Leukemia and Lymphoma Society
The Leukemia and Lymphoma Society
01.1999 - 01.2003
- Mentor: Dr. Rudi Grosschedl, Professor. Gene Center and Institute of Biochemistry, University of Munich.
Postdoctoral Fellow
University of Missouri
01.1998 - 01.1999
- Mentor: Dr. Mark Hannink, Professor. Biochemistry Department. University of Missouri.
Graduate Research Assistant
University of Missouri
01.1993 - 01.1998
- Biochemistry Department, School of Medicine, University of Missouri.
- School of Medicine
Graduate Research Assistant
University of Missouri
01.1991 - 01.1993
- Fisheries and Wildlife Department, School of Natural Resources, University of Missouri.
- School of Natural Resources
Education
Ph.D. - Biochemistry
University of Missouri
Columbia, MO01-1998
M.S. - Fisheries and Wildlife
University of Missouri
Columbia, MO01-1993
B.S. - Biology
McMaster University
Hamilton, Ontario, Canada01-1990
Skills
- Laboratory techniques
- Teaching
- Training and mentoring
- Documentation skills
SELECTED AWARDS AND PROFESSIONAL AFFILIATIONS
Awards, 1998: Superior Graduate Achievement Award, 1995: Donald K. Anderson Graduate Student Teaching Award, 1993: Who’s Who in America, Professional Affiliations, 1998: Sigma Xi Honorary Scientific Society, 1997: Phi Lambda Upsilon Honorary Chemistry Society
PUBLICATIONS
- 1. Y.-L. Liao, J.-R. Yang, H.-Y. Kuo, Y.-S. Chen, Y.-C. Lin, S. Sachdev, D-Y. Kim, R. M. Roberts, Y. Yuan, and T. Ezashi. Improving the differentiation capability of porcine induced pluripotent stem cells by using the additional reprogramming factor TBX3. FEBS Letters. In press.
- 2. Gillespie, S. W., A. S. Reddy, S. Sachdev, N. Nagvi, C. L. Lorson, and K. Singh. 2023. Islatravir (MK-8591): Evaluation of clinical development for HIV and HBV. Submitted.
- 3. Kinkade, J. A., A. S. Seetharam, S. Sachdev, N. J. Bivens, B. S. Phinney, G. Grigorean, R. M. Roberts, G. Tuteja, and C. S. Rosenfeld. 2023. Extracellular vesicles from mouse trophoblast cells: effects on neural progenitor cells and potential participants in the placenta-brain Axis. Biol Reprod. Oct 26.
- 4. Byaredy. S. N., K. Sharma, S. Sachdev, A. S. Reddy, A. Acharya, K. M. Klaustermeier, C. L. Lorson, and K. Singh. 2023. Potential therapeutic targets for Mpox: the evidence to date. Expert Opin Ther Targets. Jan-Jun;27(6):419-31.
- 5. Spratt A. N., S. R. Kannan, K. Sharma, S. Sachdev, S. L. Kandasamy, A. Sönnerborg, C. L. Lorson, and K. Singh. 2022. Continued Complexity and Evolution of Omicron Mutations Biomedicines. Oct 16;10(10):2593
- 6. Kannan S. R.
- S. Sachdev
- A. S. Reddy, S. L. Kandasamy, S. N. Byaredy, C. L. Lorson, and K. Singh. 2022. Mutations in the monkeypox virus replication complex: Potential contributing factors to the 2022 Outbreak. J. Autoimmun. 133, December 2022.
- Equal contribution.
- 7. Shanbhag V., S. Sachdev, J. A. Flores, M. J. Modak, and K. Singh. 2018. Family A and B DNA Polymerases in Cancer: Opportunities for Therapeutic Interventions. Biology (Basel). Jan 27(1):5.
- 8. Roeseler D. A., S. Sachdev, D. M. Buckley, T. Joshi, D. K. Wu, D. Xu, M. Hannink, and S. T. Waters. 2012. Elongation factor 1 alpha1 and genes associated with Usher syndrome are downstream targets of GBX2. PLoS One. 7(11): e4736
- 9. Roeseler D. A., S. Sachdev, T. Joshi, C. H. Hwang, D. Xu, M. Hannink, and S. Waters. GBX2 target gene identification reveals Usher Syndrome genes PCD15 and USH2A. 2011. J Nurse-Midwifery 356(1):351.
- 10. Roberts D., U. V. Pedmale, J. Morrow, S. Sachdev, E. Lechner, X. Tang, N. Zheng, M. Hannink, P. Genschik, and E. Liscum. 2011. Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3(NPH3). Plant Cell. ct;23(10):3627-40.
- 11. Cirak S., F. Von Deimling, S. Sachdev, W. J. Errington, R. Herrmann, G. Osse, C. Bonnemann, K. Brockmann, S. Hinderlich, T. H. Lindner, A. Steinbrecher, K. Hoffmann, G. G. Prive, M. Hannink, P. Nurnberg, and T. Voit. 2010. Kelk-like homolog 9 mutation is associated with an early onset autosomal dominant distal myopathy. Brain 2010 July;133(Pt7):2123-35. Comment in Brain 2010 July:133(Pt7):1865-8.
- 12. Powell, M. D., G. Manandhar, L. Spate, M. Sutovsky, S. Zimmerman, S. Sachdev, M. Hannink, J. Green, R. S. Prather, and P. Sutovsky. 2010. Discovery of putative oocyte quality markers by Exac Tag proteomics. Prot. Clin. Apps. 4:337-51.
- 13. Ezashi T., B. P. Telugu, A. Alexenko, S. Sachdev, S. Sinha and R. M. Roberts. 2009. Derivation of induced pluripotent stem cells from pig somatic cells. Proc. Natl. Acad. Sci. USA. 106:10993-8.
- 14. Westfall, S. D., S. Sachdev, P. Das, L. B. Hearne, M. Hannink, R. M. Roberts, and T. Ezashi. 2008. Identification of oxygen-sensitive transcriptional programs in human embryonic stem cells. Stem Cells Dev. 17:869-82.
- 15. Srinivasan, R., S-W. Jang, R. M. Ward, S. Sachdev, T. Ezashi, and J. Svaren. 2007. Regulation of NAB corepressor expression in Schwann cells. BMC Mol. Biol. 8:117.
- 16. Roberts, R. M., T. Ezashi, S. Westfall, S. Sachdev, and M. Hannink. 2006. Epigenetics and embryonic stem cells (Chapter 18) in Jinglun Xue (Ed) Epigenetics: Principles, Protocols and Practice. Shanghai Science and Technology Publishers, Inc. pp.273-281 & pp.355-372.
- 17. Ghosh, D., S. Sachdev, M. Hannink, and R. M. Roberts. 2005. Coordinate regulation of basal and cAMP-activated expression of hCGα by Ets2 and CREB. Mol. Endocr. 19:1049-66.
- 18. Sachdev, S., L. K. Bruhn, H. Sieber, A. Pichler, F. Melchior, and R. Grosschedl. 2001. PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies^. Genes Dev. 15:3088-3103. Comment in Genes Dev. 2001 Dec 1;15(23):3053-8.
- 19. Sachdev, S., S. Bagchi, D. D. Zhang, A. Mings, and M. Hannink. 2000. Nuclear import of IκBα is accomplished by a Ran-independent transport pathway. Mol. Cell. Biol. 20:1571-1582
- 20. Sachdev, S. and M. Hannink. 1998. Loss of IκBα-mediated control over nuclear import and DNA-binding enables oncogenic activation of c-Rel. Mol. Cell. Biol. 18:5445-5456.
- 21. Sachdev, S., A. Hoffmann, and M. Hannink. 1998. Nuclear localization of IκBα is mediated by the second ankyrin repeat: the IκBα ankyrin repeats define a novel class of cis-acting nuclear import sequences. Mol. Cell. Biol. 18:2524-2534.
- 22. Sachdev, S., J. A. Diehl, T. A. McKinsey, A. Hans, and M. Hannink. 1997. A threshold nuclear level of the v-Rel oncoprotein is required for transformation of avian lymphocytes. Oncogene 14:2585-2594.
- 23. Rottjakob, E. M., S. Sachdev, C. A. Leanna, T. A. McKinsey, and M. Hannink. 1996. PEST-dependent cytoplasmic retention of v-Rel by IκB-α: Evidence that IκB-α regulates cellular localization of c-Rel and v-Rel by distinct mechanisms. J. Virology 70:3176-3188.
- 24. Sachdev, S., E. M. Rottjakob, J. A. Diehl, and M. Hannink. 1995. IκB-α-mediated inhibition of nuclear transport and DNA-binding by Rel proteins are separable functions: Phosphorylation of C-terminal serine residues of IκB-α is specifically required for inhibition of DNA-binding. Oncogene 11:811-823.
GRANTS AND FELLOWSHIPS
- Sachdev, S. 2017. Monitoring polymerase α complex in the absence and presence of Minute Virus of Mice. Molecular Cytology Core and Office of Research, University of Missouri. $1,450 awarded.
- Sachdev, S. 1999-2002. Regulation of the nuclear-cytoplasmic distribution of LEF1. The Leukemia and Lymphoma Society. $99,750 awarded.
- Noltie, D. B. and S. Sachdev. 1991. Assessing the genetic relatedness of putative species of Missouri Amblyopites. University of Missouri Research Council. $5,000 awarded.
TEACHING EXPERIENCE
- 2017: Co-instructor of Advanced Virology Class, University of Missouri.
- 2003-2018: Co-instructor of Health Science 1000 - Introduction to Health Professions, University of Missouri.
- 2003-2018: Co-instructor of GH1010 – Career Explorations in the Health Professions, University of Missouri.
- 2002: Co-organizer and instructor of Advanced Biochemistry Laboratory Course. Gene Center and Institute of Biochemistry, University of Munich.
- 2001: Co-organizer and instructor of EMBO course on Protein-Protein Interactions. Gene Center and Institute of Biochemistry, University of Munich.
- 1999-2017: Mentoring of undergraduate and graduate students. Gene Center and Institute of Biochemistry, University of Munich and University of Missouri, Bond Life Sciences Center.
- 1994-1995: Teaching Assistant, Molecular Biology Laboratory Course. Biochemistry Department, University of Missouri.
- 1990-1991: Teaching Assistant, Biology Laboratory Course. Biology Department, McMaster University.
- 1988-1990: French Monitor. Assisting with teaching high school students about the French language and culture. Hamilton Board of Education and Ontario Ministry of Education.
PUBLISHED ABSTRACTS
- Sachdev, S., and M. Hannink. 1997. The ankyrin repeat domain of IκB-α mediates nuclear shuttling: reduced control by IκB-α over nuclear localization of Rel proteins contributes to oncogenic activation. FASEB J. 11: A1066.
- Sachdev, S., K. C. Cone, and D. B. Noltie. 1992. Molecular analysis of mtDNA as a tool for classifying members of the genus Amblyopites (Pisces: Centrarchidae). Accepted for presentation at the Genetics and Evolution of Aquatic Organisms meeting.
PRESENTATIONS
- Invited Seminars
- Sachdev, S. May 2001. Negative regulation of LEF-1/TCF and Wnt signaling by PIASy. Biochemistry Department, University of Missouri.
- Oral Presentations at International Meetings
- Sachdev, S., L. K. Bruhn, H. Sieber, and R. Grosschedl. 2001. Negative regulation of LEF-1/TCF by PIASy. The Wnt Meeting 2001 (New York, NY).
- Sachdev, S., and M. Hannink. 1998. Nuclear localization of IκBα is mediated by the second ankyrin repeat: Loss of IκBα-mediated inhibition of nuclear import and DNA-binding enables oncogenic activation of c-Rel. Fourteenth Annual Meeting on Oncogenes (San Diego, CA).
- Sachdev, S., and M. Hannink. 1996. Role of nuclear export in regulation of cellular localization and oncogenicity of v-Rel. Twelfth Annual Meeting on Oncogenes (Frederick, MD).
- Sachdev, S., E. M. Rottjakob, J. A. Diehl, and M. Hannink. 1995. C-terminal phosphorylation of IκBα defines inhibition of nuclear translocation and inhibition of DNA-binding as separable functions. Eleventh Annual Meeting on Oncogenes (Frederick, MD).
- Sachdev, S., K. C. Cone, J. B. Koppelman, and D. B. Noltie. 1993. Classification of Missouri Amblyopites populations by molecular analysis of mtDNA genes. University of Missouri, The School of Natural Resources Fisheries and Wildlife Departmental Seminar (Columbia, MO).
- Sachdev, S., K. C. Cone, and D. B. Noltie. 1993. Restriction Fragment Length Polymorphisms in the ND2 gene as a tool for classifying populations of Missouri Amblyopites (Pisces: Centrarchidae). University of Missouri 10th Annual Research and Creative Activities Forum (Columbia, MO).
- Sachdev, S., K. C. Cone, and D. B. Noltie. 1992. Restriction Fragment Length Polymorphisms in the ND2 gene as a tool for classifying populations of Missouri Amblyopites (Pisces: Centrarchidae). Division of Biological Sciences Retreat (Lake of the Ozarks, MO).
- Sachdev, S., K. C. Cone, and D. B. Noltie. 1992. Restriction Fragment Length Polymorphisms in the ND2 gene as a tool for classifying populations of Missouri Amblyopites (Pisces: Centrarchidae). Genetics and Evolution of Aquatic Organisms (Bangor, Wales).
- Sachdev, S., K. C. Cone, and D. B. Noltie. 1992. Restriction Fragment Length Polymorphisms in the ND2 gene as a tool for classifying populations of Missouri Amblyopites (Pisces: Centrarchidae). 54th Midwest Fish and Wildlife Conference (Toronto, ON).
- Poster Presentations at Meetings
- Coy, M., S. Sachdev, L. C. Rogers, I. Naqvi, A. A. Edwards, D. H. Burke, J. J. Cannon, A. Sonnerberg, U. Neogi, and K. Singh. 2022. Molecular mechanism of N155H/E92Q resistance to HIV-1 integrase inhibitors. Great Plains Infectious Disease Meeting (Columbia, MO).
- Sachdev, S., and M. Hannink. 1998. Nuclear localization of IκBα is mediated by the second ankyrin repeat: Loss of inhibition of nuclear import by IκBα contributes to v-Rel-mediated oncogenic transformation. Dynamic Organization of the Nucleus (Cold Spring Harbor, NY).
- Sachdev, S., and M. Hannink. 1998. Nuclear localization of IκBα is mediated by the second ankyrin repeat: Loss of inhibition of nuclear import by IκBα contributes to v-Rel-mediated oncogenic transformation. Molecular Biology Week ’98, University of Missouri (Columbia, MO).
- Sachdev, S., and M. Hannink. 1997. Identification of a novel nuclear transit sequence in IκBα: Loss of control by IκBα over nuclear localization of Rel proteins contributes to oncogenic activation. Symposium on the Eukaryotic Nucleus (Airlie, VA).
- Sachdev, S., and M. Hannink. 1997. The ankyrin repeat domain of IκB-α mediates nuclear shuttling: reduced control by IκB-α over nuclear localization of Rel proteins contributes to oncogenic activation. Seventeenth International Congress of Biochemistry and Molecular Biology in conjunction with 1997 Annual Meeting of the American Society for Biochemistry and Molecular Biology (San Francisco, CA).
- Sachdev, S., and M. Hannink. 1997. The ankyrin repeat domain of IκB-α mediates nuclear shuttling: Reduced control by IκB-α over nuclear localization of Rel proteins contributes to oncogenic activation. International Union of Biochemistry and Molecular Biology and American Society for Biochemistry and Molecular Biology Young Scientists’ Program (Asilomar, CA).
- Sachdev, S., and M. Hannink. 1996. IκB-α regulates the cellular localization of c-Rel and v-Rel by distinct mechanisms. Molecular Biology Week ’96, University of Missouri (Columbia, MO).
- Diehl, J. A., S. Sachdev, and M. Hannink. 1995. Multistep activation of Rel family transcription factors: Regulation of RelA nuclear translocation by phosphorylation. Eleventh Annual Meeting on Oncogenes (Frederick, MD).
- Rottjakob, E., S. Sachdev, and M. Hannink. 1995. PEST-independent and PEST-dependent interactions of p40 (IκB-α) with Rel proteins: Evidence for in vivo conformational differences between v-Rel and c-Rel. Eleventh Annual Meeting on Oncogenes (Frederick, MD).
- Sachdev, S., and M. Hannink. 1994. IκB contains multiple C-terminal phosphorylated serine residues that are required for inhibition of DNA-binding by Rel proteins. 37th Annual Biochemistry and Molecular Biology Conference (Columbia, MO).
Timeline
Senior Research Associate
University of Missouri
10.2023 - 03.2026
Senior Research Associate
University of Missouri
09.2023 - 09.2023
Senior Research Associate
University of Missouri
07.2022 - 08.2023
Senior Research Associate
University of Missouri
08.2019 - 06.2022
Senior Research Associate
University of Missouri
09.2018 - 07.2019
Senior Research Scientist
University of Missouri
07.2018 - 08.2018
Senior Research Scientist
University of Missouri
08.2011 - 07.2018
Research Scientist
University of Missouri
08.2009 - 07.2011
Postdoctoral Fellow
University of Missouri
04.2003 - 08.2009
Fellow of the American Leukemia and Lymphoma Society
The Leukemia and Lymphoma Society
01.1999 - 01.2003
Postdoctoral Fellow
University of Missouri
01.1998 - 01.1999
Graduate Research Assistant
University of Missouri
01.1993 - 01.1998
Graduate Research Assistant
University of Missouri
01.1991 - 01.1993
B.S. - Biology
McMaster University
M.S. - Fisheries and Wildlife
University of Missouri
Ph.D. - Biochemistry
University of Missouri