Sergey Grigoryev

I am an enthusiastic, high energy, professional biologist, with big experience in electrophysiology study of ion channels, manual and high throughput screening platforms. With strong interest of ion channel in investigating the structure-function and regulatory mechanisms of Ca-channels, voltage dependent potassium channel and mitochondrial protein import channels. I have big experience in working with conventional and automatic patch clamp, artificial planar lipid methods. Big experience for designing new methods and experiments for characterization, investigations and lead optimization of compounds effecting on ion channels I have hand on many research methods including confocal microscopy, molecular biology, immunology. I have very good communication skills, willing to learn new methods and equipments. I enjoy research new knowledge and like creative and challenging work.

• Biophysical Society since 2002
• American Society of the Cell Biology since 2004
• Received Industry Certificate from the Life Science Immersion Program (LSIP) of the BIOCOM Institute for transition from academia to industry and move up the ladder in industrial careers (2012)
• Society of Neuroscience since 2018

PUBLICATIONS:

1) Pakhomov AG, Grigoryev S, Semenov I, Casciola M, Jiang C, Xiao S. The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency. Bioelectrochemistry. 2018 Mar 29;122:123-133

2) Gavriliouk D, Scrimgeour NR, Grigoryev S, Ma L, Zhou FH, Barritt GJ, Rychkov GY. Regulation of Orai1/STIM1 mediated ICRAC by intracellular pH. Sci Rep. 2017 Aug 29;7(1):9829

3) Muratori C, Pakhomov AG, Heller L, Casciola M, Gianulis E, Grigoryev S, Xiao S, Pakhomova ON. Electrosensitization Increases Antitumor Effectiveness of Nanosecond Pulsed Electric Fields In Vivo. Technol Cancer Res Treat. 2017 Jan 1:1533034617712397.

4) Wen L, Voronina S, Javed MA, Awais M, Szatmary P, Latawiec D, Chvanov M, Collier D, Huang W, Barrett J, Begg M, Stauderman K, Roos J, Grigoryev S, Ramos S, Rogers E, Whitten J, Velicelebi G, Dunn M, Tepikin AV, Criddle DN, Sutton R. Inhibitors of ORAI1 Prevent Cytosolic Calcium-Associated Injury of Human Pancreatic Acinar Cells and Acute Pancreatitis in 3 Mouse Models. Gastroenterology. 2015 Aug;149(2):481-492.

5)  Santos JS, Grigoriev SM, Montal M. Molecular template for a voltage sensor in a novel K+ channel. III. Functional reconstitution of a sensorless pore module from a prokaryotic Kv channel. J Gen Physiol. 2008 Dec;132(6):651-6.

6) Martinez-Caballero S, Grigoriev SM, Herrmann JM, Campo ML, Kinnally KW. Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23. J Biol Chem. 2007 Feb 9;282(6):3584-93.

7) Pavlov E, Grigoriev SM, Dejean LM, Zweihorn CL, Mannella CA, Kinnally KW. The mitochondrial channel VDAC has a cation-selective open state. Biochim Biophys Acta. 2005 Dec 20;1710(2-3):96-102.

8) Chinopoulos C, Starkov AA, Grigoriev S, Dejean LM, Kinnally KW, Liu X, Ambudkar IS, Fiskum G. Diacylglycerols activate mitochondrial cationic channel(s) and release sequestered Ca(2+). J Bioenerg Biomembr. 2005 Aug;37(4):237-47.

9) Grigoriev, S.M., Muro, C., Dejean, L.M., Campo, M.L., Martinez-Caballero, S., Kinnally, K.W. (2004) Electrophysiological approaches to the study of protein translocation in mitochondria. Int Rev Cytol. 2004; 238:227-74. Review.

10)  Mironova GD, Negoda AE, Marinov BS, Paucek P, Costa AD, Grigoriev SM, Skarga YY, Garlid KD Functional distinctions between the mitochondrial ATP-dependent K+ channel (mitoKATP) and its inward rectifier subunit (mitoKIR). J. Biol Chem. 2004 May 11.

11)  Guo L, Pietkiewicz D, Pavlov EV, Grigoriev SM, Kasianowicz JJ, Dejean LM, Korsmeyer SJ, Antonsson B, Kinnally KW. Effects of cytochrome c on the mitochondrial apoptosis-induced channel MAC. Am J Physiol Cell Physiol. 2004 May;286(5):C1109-17. Epub 2003 Dec 24.

12)  Grigoriev SM, Jensen RE, Kinnally KW. Control of mitochondrial protein import by pH. FEBS Lett. 2003 Oct 9;553(1-2):163-6.

13)  Muro, C., Grigoriev S.M., Pietkiewicz, D., Kinnally, K.W., and Campo, M.L. Comparison of the Tim and Tom channel activities of the mitochondrial protein import complexes. Biophysical J. 2003 May;84(5):2981-9.

14)  Marinov BS, Grigoriev SM, Skarga YuYu, Olovjanishnikova GD, Mironova GD. Effects of pelargonidine and a benzocaine analogue p-diethylaminoethyl benzoate on mitochondrial K(ATP) channel. Membr Cell Biol. 2001 Jul;14(5):663-71

15)  Mironova GD, Skarga YY, Grigoriev SM, Negoda AE, Kolomytkin OV, Marinov BS. Reconstitution of the mitochondrial ATP-dependent potassium channel into bilayer lipid membrane. Journal of Bioenergetics and Biomembranes, 1999, 31(2):157-61

16)  Grigoriev SM, Skarga YY, Mironova GD, Marinov BS. Regulation of mitochondrial KATP channel by redox agents Biochim Biophys Acta, 1999, 1410(1):91-6

17)  Mironova GD, Grigoriev SM, Skarga YuYu, Negoda AE, Kolomytkin OV. ATP-dependent potassium channel from rat liver mitochondria: inhibitory analysis, channel clusterization. Membr Cell Biol 1997;10(5):583-91

18)  Mironova GD, Skarga YuYu, Grigoriev SM, Negoda AE, Kolomytkin OV. ATP-dependent potassium channel from rat liver mitochondria.1. Isolation, purification, and reconstitution in a bilayer lipid membrane. Membr Cell Biol 1996;10(4):429-37