Razieh Kamali
Coquitlam,BC
Summary
Highly skilled Transmission Electron Microscopy (TEM) and Cryo-EM specialist with a Ph.D. in Biochemistry from the University of Alberta and over five years of hands-on expertise in protein structure, molecular biology, and electron microscopy. Proven ability to design, plan, and execute complex experiments in dynamic and collaborative environments.
Overview
14
14
years of professional experience
Work History
Maternity Leave
08.2023 - Current
- Managed relocation to British Columbia while continuing to engage in professional development through online courses
- Maintained active connections with the scientific community to stay updated on industry advancements.
Postdoctoral Fellow, Cryo-TEM Facility Technician
Centre for Prions & Protein Folding Diseases (CPPFD) - University of Alberta
4 2021 - 8 2023
- Provided maintenance, user support, and training for the Cryo-TEM facility
- Conducted structural studies of recombinant and brain-derived prion fibrils, tau fibrils, and misfolded proteins associated with other neurodegenerative disorders
- Performed protein analysis experiments and authored a review manuscript on the structural characterization of prion proteins.
Graduate Research Assistant
Department of Biochemistry - University of Alberta
01.2015 - 01.2021
- Received training in cryo-EM and single particle 3D reconstruction
- Performed negative staining and cryo-EM on recombinant and brain-derived prion proteins
- Conducted three-dimensional reconstruction of BSE prion fibrils utilizing image processing techniques
- Developed a protocol for immunogold labeling using electron microscopy
- Conducted experiments in protein purification, gradient centrifugation, Western blotting, SDS-PAGE, PCR, and bioassays
- Authored five manuscripts and contributed to the grant writing process
- Presented research findings at national and international conferences
- Mentored team members and collaborated on various projects.
Teaching Assistant
Medical Microbiology & Immunology & Biochemistry Departments - University of Alberta
01.2015 - 01.2019
- Assisted in the development and delivery of course materials, including lectures and assignments
- Provided support to students by conducting review sessions, answering questions, and offering guidance on coursework.
Laboratory Technician/Supervisor
Marlik Medical Diagnostic Laboratory
01.2013 - 01.2014
- Conducted diagnostic tests in various fields and supervised laboratory staff
- Managed equipment maintenance and sample processing.
Research Assistant
Pasteur Institute- Influenza & Respiratory Diseases Department
01.2011 - 01.2013
- Conducted cell culture, virus titration, gene cloning, and protein purification experiments
- Contributed to vaccine development projects.
Education
Ph.D. in Biochemistry - Specialized in the structural investigation of misfolded prion protein and tau protein using TEM.
University of Alberta
Skills
Laboratory Techniques: Protein purification, differential centrifugation, molecular cloning, ELISA, PCR, immunogold labeling
Protein Analysis: Western blot, SDS-PAGE, silver staining
Microscopy: Transmission electron microscopy (TEM) operation and troubleshooting, Negative staining, Cryo-EM
Software: MS Office, EMAN, Spider, iMagic
Certificatesandtraining
- Single particle and helical 3D reconstruction
- Cryo-EM training- FEI Company
- Concepts in Biosafety: University of Alberta Personnel
- Workplace Hazardous Material Information System (WHMIS)
- Chemical Safety: University of Alberta Personnel
- Graduate Teaching and Learning Program Level 1 and 2 Completion, University of Alberta
Publications
- Implication of exosomes derived from cholesterol accumulated astrocytes in Alzheimer's disease pathology., Disease Models & Mechanisms, 14, 10, dmm048929, 10/2021
- The ultrastructure of infectious L-type bovine spongiform encephalopathy prions constrains molecular models., PLoS Pathog, 17, 6, e1009628, 06/2021
- Seizures are a druggable mechanistic link between TBI and subsequent tauopathy., Elife, 2020.05.091819, 2020
- Isolation of infectious, non-fibrillar and oligomeric prions from a genetic prion disease., Brain, 143, 5, 1512-1524, 2020
- The CNS in inbred transgenic models of 4-repeat Tauopathy develops consistent tau seeding capacity yet focal and diverse patterns of protein deposition., Molecular Neurodegeneration, 12, 1, 72, 2017
- The effect of various stabilizers on preserving immunogenicity of lyophilized mumps vaccine, J Res Health Sci., 17, 4, e00393, 2017
- HIV-1 Vpr reactivates latent HIV-1 provirus by inducing depletion of class I HDACs on chromatin, Scientific Reports, 6, 31924, 2016
- Application of Real-Time PCR method for evaluation of measles vaccine heat stability, Journal of Paramedical Sciences (JPS), 6, 2, 2015
- Evaluation of the thermal stability of a novel strain of live-attenuated mumps vaccines (RS-12 strain) lyophilized in different stabilizers, Journal of Virological Methods, 199, 35-38, 2014
References
- Dr. Holger Wille, Professor of Biochemistry, University of Alberta, wille@ualberta.ca, (780) 248-1712, Ph.D. Supervisor
- Dr. Howard Young, Professor of Biochemistry, University of Alberta, hyoung@ualberta.ca, (780) 492-4577, Supervisory Committee
- Dr. Michael Overduin, Professor of Biochemistry, University of Alberta, overduin@ualberta.ca, (780) 248-1186, Supervisory Committee
Timeline
Maternity Leave
08.2023 - Current
Graduate Research Assistant
Department of Biochemistry - University of Alberta
01.2015 - 01.2021
Teaching Assistant
Medical Microbiology & Immunology & Biochemistry Departments - University of Alberta
01.2015 - 01.2019
Laboratory Technician/Supervisor
Marlik Medical Diagnostic Laboratory
01.2013 - 01.2014
Research Assistant
Pasteur Institute- Influenza & Respiratory Diseases Department
01.2011 - 01.2013
Postdoctoral Fellow, Cryo-TEM Facility Technician
Centre for Prions & Protein Folding Diseases (CPPFD) - University of Alberta
4 2021 - 8 2023
Ph.D. in Biochemistry - Specialized in the structural investigation of misfolded prion protein and tau protein using TEM.
University of Alberta
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