Ragini Ganesha Rao
Fergus,ON
Summary
Organized and dependable candidate successful at managing multiple priorities with a positive attitude. Willingness to take on added responsibilities to meet team goals. To seek and maintain full-time position that offers professional challenges utilizing interpersonal skills, excellent time management and problem-solving skills.
Overview
21
21
years of professional experience
Work History
Post, Punjabi University
01.1999
- Research title: Isolation of Bacteriocin producing microorganism from natural source using zone inhibition assay
Research Scientist
Alchem International Ltd
10.2007 - 01.2010
- Technology transfer of Streptokinase-includes microbial production of streptokinase at pilot scale (100 Lt) and its scaling up (~3000 Lt), Recovery of protein and its down stream processing using centrifugation, cross flow microfiltration & ultrafiltration, Ion exchange chromatography, standardizing streptokinase assay procedure, Preparation of Lab Instructions, Standard Test Protocols etc
- For the technology
- Demethylecolchicine- isolation and strain improvement of microorganism for the biotransformations, media optimization at flask level and its scaling up in pilot scale Aplikon fermenter, preparation of Lab Instructions
- Ceramides: Initiated project on Ceramide – a cosmeceutical product
Carbohydrate and Protein, Punjabi University
01.2003 - 01.2008
- Title: Development of a Continuous System for the Preparation of High Fructose Syrup from inulin by immobilized inulinase
- Optimization of component and process parameters for enzyme production at flask as well as bench top bioreactor level
- Purification of protein using various salt/ solvent precipitation and chromatographic techniques (Ion exchange, Gel exclusion)
- Immobilization of enzyme using different techniques (physical adsorption, crosslinking, ionic and covalent bonding)
- Kinetic characterization and comparison of free and immobilized enzyme
- Development of a PBR (Packed Bed Reactor) for continuous system
- Biochemical analysis of carbohydrates, proteins.
Production Manager
APIS NATURAL Product Pvt. Ltd
02.2002 - 10.2002
- Production planning
- Employment and training of subordinates
- Creating Standard Operating Procedures
- Future technical development and improvement in production.
Team Member
Tim Hortons
09.2022 - Current
- Learned all required tasks quickly to maximize performance.
- Worked scheduled shifts and remained available to work during coworker absences, holidays, and busy periods.
- Contributed to team success by completing jobs quickly and accurately.
- Developed strong cooperative relationships with coworkers and managers.
Education
PG Diploma - Clinical Research
Academy of Applied and Pharmaceutical Sciences
2011
Ph.D - Life Sciences, Enzyme and Fermentation Technology
Punjabi University
2008
M.Sc - Microbial & Food Technology
Punjabi University
1999
B.Sc - Home science
Govt. College for women
1996
Skills
- Biochemical technology: Chromatographies, Ultra and microfiltration, SDS-PAGE, GC, HPLC, Spectrophotometry, biochemical assays (eg protein, carbohydrates )
- Microbiology: Basic microbiological handling and laminar air flow bench working, Isolation, strain improvement and preservation of culture, isolation and preservation mutation and protoplast fusion, Hand-on training on R-DNA technology, biomass production at flask as well as pilot scale fermenter
Writing: Experienced in writing documentation in laboratory like Standard Operating Procedure, Lab instructions as well as Research papers
Accomplishments
1. Singh, R.S., Dhaliwal, R. and Puri, M. (2006). Production of inulinase from Kluyveromyces marxianus YS-1 using root extract of Asparagus racemosus. Process Biochem. 41: 1703-1707.
2. Singh, R.S., Dhaliwal, R. and Puri, M. (2006). Partial purification and characterization of exoinulinase from Kluyveromyces marxianus YS-1 for preparation of high fructose syrup. J. Microbiol. Biotechnol. 17(5) 733-8
Timeline
Team Member
Tim Hortons
09.2022 - Current
Research Scientist
Alchem International Ltd
10.2007 - 01.2010
Carbohydrate and Protein, Punjabi University
01.2003 - 01.2008
Production Manager
APIS NATURAL Product Pvt. Ltd
02.2002 - 10.2002
Post, Punjabi University
01.1999
PG Diploma - Clinical Research
Academy of Applied and Pharmaceutical Sciences
Ph.D - Life Sciences, Enzyme and Fermentation Technology
Punjabi University
M.Sc - Microbial & Food Technology
Punjabi University
B.Sc - Home science
Govt. College for women
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