Praveen Kumar Guttula

Postdoctoral Fellow with two years in fellowship Ottawa Hospital and Research Institute. Organized with research and presentations educating students, peers and superiors on research analysis. Published research findings through peer-reviewed articles and analyzed results tailoring to possible future applications.

• D. No 3-173, Gandhinagar Colony, PG Peta, Srikakulam, 532401, India
• 501 Smyth Road, Ottawa, Ontario, K1H 8L6, Canada

• Large-Scale Bioinformatics Challenges
• Genomic Analysis Innovations
• Computational Systems Biology
• Drug Discovery and Molecular Design
• Cancer Biology

• Identification and validation of putative biomarkers by in silico analysis, mRNA expression and oxidative stress indicators for negative energy balance in buffaloes during transition period, Savleen Kour, Neelesh Sharma, PK Guttula, Mukesh Kumar Gupta, Marcos Veiga Dos Santos, Goran Bacic, Nino Macesic, Anand Kumar Pathak, Young-Ok Son, Animal Bioscience, 37, 3, 522, 2024, 2.2
• Multimodal Imaging of Pancreatic Cancer Microenvironment in Response to an Antiglycolytic Drug, Elnaz Sheikh, Kirti Agrawal, Sanjit Roy, David Burk, Fabrizio Donnarumma, Young H Ko, PK Guttula, Nrusingh C Biswal, Hem D Shukla, Manas Ranjan Gartia, Advanced Healthcare Materials, 12, 31, 2301815, 2023, 10
• Restoration of Altered Oncogenic and Tumor Suppressor microRNA Expression in Breast Cancer and Colorectal Cancer Cell using Epicatechin, S Kiran, A Patra, P Verma, S Purkait, G Chhabra, PK Guttula, A Ghosh, Current Molecular Pharmacology, 16, 8, 915-926, 2023, 2.7
• Unveiling the anticancer mechanism of 1, 2, 3-triazole-incorporated thiazole-pyrimidineisoxazoles: insights from docking and molecular dynamics simulations, Reddymasu Sireesha, Y Pavani, Bala Divya Mallavarapu, Bilal Ahmed Abbasi, PK Guttula, Mannam Subbarao, Journal of Biomolecular Structure and Dynamics, 1-13, 2023, 5.235
• Analyzing the effect of heparin on in vitro capacitation and spermatozoal RNA population in goats, Bijayalaxmi Sahoo, Balaram Mishra, Rakesh Bhaskar, YNV Vikas, Anushri Umesh, PK Guttula, Mukesh Kumar Gupta, International Journal of Biological Macromolecules, 241, 124502, 2023, 8.2
• Prediction of potential molecular markers of bovine mastitis by meta-analysis of differentially expressed genes using combined p value and robust rank aggregation, A Umesh, PK Guttula, MK Gupta, Tropical Animal Health and Production, 54, 5, 269, 2022, 1.7
• Molecular modeling, docking and dynamic simulations of growth hormone receptor (GHR) of Labeo rohita, Bhushan C Dhandare, Mohd Ashraf Rather, BP Bhosale, Ravindra Pawar, PK Guttula, AU Pagarkar, Journal of Biomolecular Structure and Dynamics, 40, 7, 3024-3037, 2022, 5.235
• Endophyte fungal isolate mediated biogenic synthesis and evaluation of biomedical applications of silver nanoparticles, Hemashekhar Bagur, Raja Sekhar Medidi, Prathap Somu, PW Jayakumar Choudhury, Chetan Shekhar Karua, PK Guttula, Govindappa Melappa, Chandrappa Chinna Poojari, Materials Technology, 37, 3, 167-178, 2022, 3.1
• Analysis of overexpressed miRNA in circulation and cancer tissue to develop a potential microRNA panel for the diagnosis of colorectal cancer, Babita Pruseth, Amit Ghosh, Dibyabhaba Pradhan, Suvendu Purkait, PK Guttula, MicroRNA, 10, 4, 250-262, 2021
• Comparison of spermatozoal RNA extraction methods in goats, B Sahoo, PK Guttula, MK Gupta, Analytical Biochemistry, 114059, 2020, 3.365
• Molecular modeling and co-expression analysis of human stem cell factor as fusion partner to granulocyte colony stimulating factor for improving their bioactivity, Bijayalaxmi Sahoo, Sagarika Dash, Srimeenakshi Sankarnarayanan, Balaram Mishra, PK Guttula, Rakesh Bhaskar, Mukesh Kumar Gupta, Journal of Biomolecular Structure and Dynamics, 1-15, 2020, 5.235
• Molecular modeling, docking and dynamic simulations of growth hormone receptor (GHR) of Labeo rohita, BC Dhandare, MA Rather, BP Bhosale, R Pawar, PK Guttula, Journal of Biomolecular Structure and Dynamics, 1-14, 2020, 5.235
• Putative biomarkers for early detection of mastitis in cattle, Zul I Huma, Neelesh Sharma, Sarabpreet Kour, Suhasani Tandon, PK Guttula, Savleen Kour, Amit Kumar Singh, Rajiv Singh and Mukesh Kumar Gupta, Animal Production Science, 60, 14, 1721-1736, 2020, 1.410
• Structural analysis, molecular docking and molecular dynamics simulations of G-protein-coupled receptor (Kisspeptin) in fish, MA Rather, S Dutta, PK Guttula, BC Dhandare, SI Yusufzai, MI Zafar, Journal of Biomolecular Structure and Dynamics, 1-20, 2020, 5.235
• Elucidating the role of Val-Asn 95 and Arg-Gly 52 mutations on structure and stability of fibroblast growth factor homologous factor 2, Nandini Sarkar, Vidyalatha Kolli, Subhankar Paul, PK Guttula, Protein and Peptide Letters, 26, 12, 2019, 1.01
• Structure based annotation of Helicobacter pylori strain 26695 proteome, Swati Singh, PK Guttula, Guruprasad Lalitha, PloS one, 9, 12, e115020, 2014, 3.240

• ICMR Senior Research Fellow (SRF) Fellowship (2018)
• ERASMUS+ exchange Fellow in IST Portugal Lisbon (2018)
• Senior Research Fellow (SRF) in DBT funded Bioinformatics Infrastructure Facility (2014)
• Qualified GATE in 2012 and 2016 and Received Fellowship from MHRD for two Years
• Best poster award in DBT sponsored seminar 'Genetic and Genome analysis for novel therapeutics' (2016)
• Qualified in APSET-2015

• UNIX/Linux
• Windows

• BWA
• STAR
• Tophat2
• StringTie
• Picard
• GATK
• VarScan
• SAMtools
• Bedtools
• VCFtools
• BAMtools
• WAnnovar
• SEURAT
• Scanpy
• Cell-Cell communication tools like Cellchat
• Modeller
• Discovery Studio
• Schrodinger
• AutoDock
• Cytoscape
• R programming
• Python
• Perl

• Post Doctoral Fellow (PDF), Ottawa Hospital Research Institute, General Campus (Regenerative Medicine), Ottawa, Canada, University of Ottawa, 06/2022, Present, Conducted Research on studying dynamics of Muscle Regeneration using single cell RNA seq data (scRNA) and integrating the data to find a Boolean logical model that explains the different states in satellite cells (Quiescent, Activated and Differentiated). Utilized tools like SEURAT Work Flow and GINSIM for data integration to develop a Boolean logical model for satellite cell states.
• Bioinformatics Scientist, Tata Medical Center, Kolkata, India, 06/2021, 04/2022, Analysis of Acute Myeloid Leukemia (AML) samples (NGS Data) for the identification of mutations. Variant analysis (DNA-seq data analysis) to find the mutations associated with Acute Myeloid Leukemia. RNA seq data analysis of control samples and AML samples to identify the Biomarkers. Survival analysis of the Cancer data using R Programming (in house data).
• Indian Council of Medical Research (ICMR) Senior Research Fellow (SRF), National Institute of Technology, Rourkela, India, 11/2018, 11/2020, Conducted Research on Project entitled 'Molecular Mechanism of Pluripotency in Testicular Stem cells-An in silico Study.'
• Erasmus + Exchange Fellow, IST Portugal, Tecnico Lisboa, INESCID Lisbon, Portugal, 02/2018, 07/2018, Focused on logical modeling of Boolean Networks for understanding pluripotency in testicular stem cells (Germline stem cells to Germline pluripotent stem cells), showcasing skills in network analysis and Boolean models development using GINsim.
• DBT SRF, NIT Rourkela, Odisha, India, 10/2014, 10/2018, Worked on Project entitled 'Bioinformatics Infrastructure Facility' and played a major role in Maintaining Bioinformatics Laboratory. Research in Bioinformatics applications of Biotechnology.

• Isolation of DNA, RNA
• PCR
• Western blotting
• SDS PAGE
• Basics of cell culture
• Nanopore Sequencing

• Dynamic basis of mRNA processing in male germline stem cell pluripotency and reprogramming by alternative polyadenylation, PK Guttula, Mukesh Kumar Gupta, International Journal of Data mining and Bioinformatics, 25, 3-4, 145-160, 2021, 0.743
• Prediction and Boolean Logical Modelling of Synergistic microRNA Regulatory Networks during Reprogramming of Male Germline Pluripotent Stem Cells, PK Guttula, Mukesh Kumar Gupta, BioSystems, 104453, 2021, 1.973
• A Boolean Logical model for Reprogramming of Testes-derived male Germline Stem Cells into Germline pluripotent stem cells, PK Guttula, Pedro T Monteiro, Mukesh Kumar Gupta, Computer Methods and Programs in Biomedicine, 192, 105473, 2020, 7.027
• Examining the Co-Expression, Transcriptome Clustering and Variation using Fuzzy Cluster Network of Testicular Stem cells and Pluripotent Stem cells compared with other cell types, PK Guttula and Mukesh Kumar Gupta, Computational Biology and Chemistry, 85, 107227, 2020, 3.737
• Pharmacophore modeling coupled with Scaffold hopping to identify novel and potent Ribosomal S6 Kinase (RSK2) protein antagonists as anti-cancer agents, PK Guttula, Agrawal K, Natarajan P, Gupta MK, Journal of Biomolecular Structure and Dynamics, 38, 16, 4947-4955, 2020, 5.235
• Screening and Insilco analysis of deleterious nsSNPs (missense) in human CSF3 for their effects on Protein Structure, Stability and Function, PK Guttula, Gopalakrishnan Chandrasekaran, and Mukesh Kumar Gupta, Computational Biology and Chemistry, 82, 57-64, 2019, 3.737
• Prediction of Novel Pluripotent Proteins involved in Reprogramming of Male Germline stem cells (GSCs) into Multipotent adult Germline stem cells (maGSCs) by Network Analysis, PK Guttula, Agarwal A, Maharana U, Gupta MK, Computational Biology and Chemistry, 76, 302-309, 2018, 3.737

• Dr. Theodore J Perkins, Senior Scientist, Ottawa Hospital and Research Institute, Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada, +1613-737-8899, ext. 79795, tperkins@ohri.ca, theodore.j.perkins@gmail.com
• Dr. Xiaojian Shao, Research Officer, Digital Technologies Research Center, National Research Council of Canada, Ottawa, Canada, +1613-993-5210, Xiaojian.Shao@nrc-cnrc.gc.ca
• Dr. Mukesh Kumar Gupta, Director, ICMR- National Animal Resource Facility for Biomedical Research (NARFBR), Hyderabad, India, +9140-23480800, director-narfbr@icmr.gov.in, mukeshkgupta@gmail.com
• Dr. Pedro Tiago Monteiro, Associate Professor, INESCID, IST-Portugal, Lisbon, Portugal, +351 213100320, Pedro.Tiago.Monteiro@tecnico.ulisboa.pt
• Dr. Isabel Sa-Correia, Professor, Institute of Bioengineering and Biosciences IST-Portugal, Lisbon, Portugal, +351 21 841 90 62, isacorreia@tecnico.ulisboa.pt
• Dr. Lalitha Guruprasad, Professor, School of Chemistry, University of Hyderabad, Hyderabad, India, +919949093712, lalitha.guruprasad@gmail.com

• Multi omics approach for biomarker development
• Nanopore Sequencing: Adventures in Metagenomics and Antimicrobial Resistance
• Data Science in Biomedical Research
• Computational Systems Biology
• Intellectual Property & Innovation Management in Knowledge Era
• Applications of Bioinformatics in Biotechnology
• Genomic and Proteomic Analysis for Developing Biosimilars