Summary

Overview

Work History

Education

Skills

Certifications Training

Timeline

Pranoti Shinde

Brampton,ON

Summary

Dedicated bioprocessing professional with hands-on experience in upstream operations and bioreactor management. Recognized for strong attention to detail, technical proficiency, and effective team collaboration, contributing to successful cell culture scaling and GMP compliance.

Overview

years of professional experience

Work History

Bioreactor Technician I

Microbix Biosystems Inc.

Mississauga, Canada

05.2024 - Current

Scaling up Vero cells to a 10.5 L bioreactor (fed-batch)
Handled Mycoplasma antigen at the flask stage.
Glass Bioreactor packaging, sterilization, inoculation, infection, and harvest.
Rubella antigen packaging, UV inactivation.
Preparation of Media, reagent formulation, flask and roller bottle passages of Vero cells.

Executive – Biosimilars Upstream Manufacturing

Reliance Life Sciences

Mumbai, India

07.2022 - 09.2022

CHO cell culture in flasks and roller bottles.
Batch record review and IPQC equipment operation.

Technical Trainee – MSAT

Stelis Biopharma

Bangalore, India

05.2021 - 06.2022

Performed tissue culture from cell revival to flask stage.
Handled equipment in IPQC: Metabolite Analyzer, Vi-Cell Analyzer, Centrifuge, Osmometer, CO2 Incubator, Biowelder and Sealer
Performed HEK cell-based upstream operations in 50 L Wave and 200 L SUBs.
Executed daily planning, validation activities, and media prep (100L).
Performed cleaning and performance validation.
Experience in IQ, OQ, and PQ of SUBs, Wave Bioreactor.

Apprentice – Upstream Bioprocessing

Zydus Cadila

Ahmedabad, India

07.2020 - 03.2021

Operated Sartorius bioreactors (10L–5,000L).
Performed media hold studies.
Reviewed Batch Manufacturing Records.
Performed CIP, SIP, FIT, and preparation of logbook and BPRs.
Conducted seed culture and inoculum development for production-scale bioreactors.