Niloufar Heydarikhorneh
Ottawa,Canada
Summary
Professional Summary
Highly skilled and dedicated biomedical research technician with 7+ years of experience in molecular biology, cell culture, and animal handling. Proficient in culturing and plating primary cells, cell lines, and stem cells, with expertise in stem cell isolation and culture. Skilled in isolating cells from various mouse organs, including smooth muscle cells from the aorta, stem cells from mouse bone marrow, generated macrophages from bone marrow-derived stem cells, and conducted mouse ECG for cardiac assessments. Proven expertise in gene cloning, PCR, protein extraction, and immunofluorescence. Proficient in preparing reagents and buffers, with strong critical thinking and data analysis skills. Effective team player with a track record of reliability and high-quality results. Skilled in handling complex issues, meeting deadlines, and adapting to changing conditions. Trilingual in Kurdish, Persian, and English, with excellent communication and problem-solving abilities. Dedicated to contributing to team success and advancing organizational objectives with enthusiasm and a willingness to learn.
Overview
6
6
years of professional experience
Work History
Biomedical Research Technician
Translational Genomic Lab, UOttawa Heart Institute
06.2019 - Current
· Collaborate with other Research Technicians to improve the company's methods
· Develop research protocols for PhD and master students
· Present findings during, local, national and international conferences
· Molecular biology assays
· Help prepare research presentations
· Supervised more than 10 graduate students and 20 undergraduate students
· Managed incoming calls, emails and faxes,
· Place the order for laboratory and work with many companies
· Manage 12 colonies of different transgenic mice line
· Isolation smooth muscle cell from aorta, and stem cell from murine bone morrow
· Proficient in immunofluorescence with professional-level expertise, including quantification of immunofluorescence signals using Harmony, Cell Profiler, and ImageJ.
· Experienced in RNAscope
· Mouse and rat dissection and tissue preparation for immunofluorescence and protein extraction
· Gene Cloning, Mini and Maxi prep
Data Entry Administrator
Emam Reza Hospital Kermanshah
09.2018 - 05.2019
- Data Entry for Correlation Between Phenotypes and Genotypes of Congenital Heart Disease
- Role and Responsibilities:
- Provided daily supervision, feedback, and oversight to data entry clerks and interns.
- Coordinated activities between the genetics laboratory and the data entry center to ensure accurate and timely data entry for 1153 patients.
- Developed comprehensive reports, presentations, and other materials to support research and analysis.
Education
M.Sc. - Medical Anatomical Science
Kermanshah University of Medical Sciences
Iran01.2018
B.Sc. - Biology
University of Chabahar
Iran01.2016
Skills
Data Analysis
Data management and analysis
Statistical analysis
Microarray data analysis
Single Cell RNA Sequencing Analysis Data
Data gathering
Animal Handling
Tissue preparation and processing
Genotyping
Breeding colony management
Subcutaneous/ Intramuscular injections
Blood collection
Laboratory Skills
Biomedical laboratory techniques
Cell culture
Sterile technique
Bioinformatics
Gene cloning
Stem cell isolation
Primer and probe design
Protein extraction
Western blotting
Immunofluorescence and Quantification of immunofluorescence signals
RNAscope
Reagent preparations
PCR and Gel electrophoresis
Immunohistochemistry
Lab equipment maintenance and calibration
Chemical handling
Hazardous waste disposal
Laboratory safety
Management and Communication
Project management
Research design
Research methodology
Experimental design
Genetics research
Inventories restock and supply
Placed orders
Qualification trial management
Materials inventory control
Breeding colony management
Written and oral communication
Effective communication
Presentation preparation
Technical writing
Recordkeeping and documentation
Publications
- Heydarikhorneh N, Vilmundarson RO, Duong A, Ho T, Keyhanian K, Soheili F, Chen HH, Stewart AF, Savior Siblings Might Rescue Fetal Lethality But Not Adult Lymphoma in Irf2bp2-Null Mice, Frontiers in Immunology, 07/04/22, 13, 868053
- Soheili Fariborz Niloufar Heydarikhormeh, Mahtab Rahbar, Bahram Nikkho, Omid Fotouhi, Abdolrahim Afkhamzadeh, Hossein Jafari & Vahid Bagheri, Human papillomavirus and its clinical relevance in oesophageal squamous cell carcinoma in a Kurdish population in the west of Iran, INFECTIOUS DISEASES, 2016, 48, 4, 270–273
- Parichehr Darabi, Fatemeh Dahmardeh, Nilofar Heydarikhorneh, Zahra Jalili, Samira Fooladi, Mohammad Saeid Hakhamanesh, and Maseoud Heidarizadeh, Nkx2-5 Mutations in Patients with Nonsyndromic Congenital Heart Disease, Zahedan Journal of Research in Medical Sciences, 01/01/16, 18, 1, e5873
Papers submitted
- Fariborz Soheili, Niloufar Heydarikhorneh, An Duong, Ragnar O. Vilmundarson, Alexandre F. R. Stewart, Bisphosphonates therapy worsen arterial calcification at MgpKO mice, Submitted to Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB), 09/25/24
- Fariborz Soheili, Niloufar Heydarikhorneh, Ragnar O. Vilmundarson, Alexandre F. R. Stewart, Mechanism whereby the chromosome 9p21.3 locus affects arterial calcification, Submitted to Cell, 10/01/24
- Niloufar Heydarikhorneh, Fariborz Soheili, Mitra Bakhtiary, Differential methylation status of H19 and MEST loci after human sperm cryopreservation from oligospermic patients, Revision submitted to Reproduction, 08/15/24
Research Projects
- Genetic Variants Mechanism of Coronary Arterial Calcification
- The Influence of Alendronate (Bisphosphonates) on Atherosclerosis Progression and Arterial Calcification in Osteoporotic Patients
- Ongoing research in the Target engagement assay development for human PTPN1
- The determination genes mutation in QT prolongation
- Comparing Efficiency of Stem Cell Isolated from Murine Bone Marrow and Compact Bone
- Genotype-Phenotype Correlation in Congenital Heart Diseases: Mutation Analysis of GATA4, NKX2.5, and TBX5
- HPV Clinical Relevance in Esophageal Squamous Cell Carcinoma Among Kurds in Western Iran
- VEGFR Gene Expression Inhibition by Chitosan-siRNA Nanoparticles in Cancer Stem Cells
- Isolation, Cultivation, and Transfection of Human Keratinocytes
- Β-Globin Gene Mutation Frequency in Baloch β-Thalassemia Patients
Published Abstracts
- Bisphosphonate therapy improves bone deficits but worsens arterial calcification in Mgp-null mice, Fariborz Soheili, PhD, MSc, Niloufar Heydarikhorneh, MSc, Benjamin Rotstein, PhD, Hsiao-huei Chen, PhD, and Alexandre Stewart, PhD, Circulation Volume 150, Number Suppl_1, 11/01/24
- Bisphosphonate therapy worsens arterial calcification at MgpKO mice, Soheili F, Heydarikhorneh N, Chen H, Stewart A, Canadian Lipid & Vascular Summit, 09/01/23
- How 9p21.3 contributes to coronary arterial calcification, Soheili F, Heydarikhorneh N, Stewart A, Cardiovascular Research Day, 04/01/23
Honors and awards
- 2023, Member of JHSC (Joint Health and Safety Committee UOHI)
- 2018, Rank 1 among all medical anatomy students at Kermanshah University of Medical Sciences
- 2016, Rank 1 at the Medical Anatomy Master Entrance Exam
- 2016, The top and first-ranked biology student, Department of Biology, Chabahar, Iran
- 2015, Chabahar Maritime University's top researcher, Department of Biology, Chabahar, Iran
Languages
English
Full Professional
Persian
Native or Bilingual
French
Elementary
Korean
Native or Bilingual
Interests
Genomics
Transcriptomics
Epigenomics
stem cell and iPSCsGenomics
Computational Biology
Cancer Genetics,
Genetics of cardiovascular diseases
Timeline
Biomedical Research Technician
Translational Genomic Lab, UOttawa Heart Institute
06.2019 - Current
Data Entry Administrator
Emam Reza Hospital Kermanshah
09.2018 - 05.2019
M.Sc. - Medical Anatomical Science
Kermanshah University of Medical Sciences
B.Sc. - Biology
University of Chabahar
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