Nathan Glonek
San Diego
Summary
Biotechnology professional with a strong foundation in microbiology, molecular biology, and immunoassays. Inquisitive and adaptable, with a strong interest in translating scientific discoveries from bench to market. Motivated to contribute across diverse areas of the biotechnology industry in fast-paced, collaborative environments.
Overview
5
5
years of professional experience
Work History
Research Associate II - Antibody Development
BioLegend
San Diego
02.2024 - Current
- Developed monoclonal antibodies against 50+ cellular, immune, and signaling protein targets, designing ELISAs, and Western blots to identify candidates with the highest commercial potential.
- Performed in vivo mouse and rat procedures, including footpad, subcutaneous, and intraperitoneal injections, and submandibular blood collection, under awake and isoflurane-anesthetized conditions.
- Contributed to the development of a rabbit recombinant monoclonal antibody development process by screening transfection supernatants, and optimizing protocols for a new platform.
- Maintained hybridoma and mammalian cell cultures (adherent and suspension), supporting assay development through the preparation of lysates, flow cytometry cells, and other screening reagents.
- Operated advanced laboratory instrumentation, including the Celigo Imaging Cytometer, plate handler™ FLEX, ClonePix2 Mammalian Colony Picker, and Nexcelom cell counters, to support experimental workflows.
- Trained and cross-trained team members in immunoassays, in vivo animal procedures, and lab instrumentation, using personal proficiency to enhance team performance, and ensure compliance with protocols.
Undergraduate Researcher
Zengler Lab, University of California, San Diego
San Diego
04.2021 - 07.2023
- Designed and executed experiments using a model rhizosphere bacterial community to study soil microbiome dynamics.
- Performed advanced microbiology techniques, including aerobic and anaerobic bacterial culturing, complex media preparation, DNA/RNA extraction, Illumina library construction, and BIOLOG microplate assays.
- Communicated research findings through weekly lab presentations and at UCSD’s 35th Annual Undergraduate Research Conference on “Constructing a Synthetic, Reproducible Consortia to Study the Soil Microbiome.”
Education
Master of Science - Biotechnology
Johns Hopkins University
Baltimore, MD12-2025
Bachelor of Science - Microbiology
University of California, San Diego
La Jolla, CA08-2023
Publication:
- Tec-Campos, D., Posadas, C., Tibocha‐Bonilla, J. D., Deepan Thiruppathy, Glonek, N., Zuñiga, C., Zepeda, A., & Zengler, K. (2023). The genome-scale metabolic model for the purple non-sulfur bacterium Rhodopseudomonas palustris Bis A53 accurately predicts phenotypes under chemoheterotrophic, chemoautotrophic, photoheterotrophic, and photoautotrophic growth conditions. PLOS Computational Biology, 19(8), e1011371–e1011371. https://doi.org/10.1371/journal.pcbi.1011371
Accomplishments
BioLegend's 2025 Q1 Employee of the Quarter: Recognized for adaptability, data analysis skills, and attitude.
Timeline
Research Associate II - Antibody Development
BioLegend
02.2024 - Current
Undergraduate Researcher
Zengler Lab, University of California, San Diego
04.2021 - 07.2023
Master of Science - Biotechnology
Johns Hopkins University
Bachelor of Science - Microbiology
University of California, San Diego