MONIKA GUPTA
Nepean,Canada
Summary
Experienced Scientist with over two years of work in the Biotech industry. Possesses a strong determination and a well-rounded skill set, consistently demonstrating expertise in Biochemistry and Molecular Biology. Sharp focus and attention to detail have led to excellence in scientific research projects. Proven track record of delivering accurate results and contributing to scientific knowledge advancement. Eager to leverage skills and contribute to future success in the field.
Overview
12
12
years of professional experience
Work History
Scientist
Novel Biotechnology Inc.
07.2023 - Current
- Design and conduct experiments with input from the assigned supervisor
- Contribute to a wide range of R&D platform development programs for nucleic acid and protein manufacturing platforms
- Maintain and operate specialized laboratory equipment, including next generation sequencing, Endotoxin testing equipment, bench-scale bioreactors and FPLCs
- Generate detailed quality data and present data supported conclusions in a timely manner in written and oral reports
- Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with assays or instrumentation.
- Trained new employees on areas of technical expertise and compliance issues relevant to lab setting.
Research Scientist
Genvira Biosciences
10.2022 - 04.2023
- Design, construct and characterize customized DNA and RNA viruses for vaccines, cancer immunotherapy and gene therapy
- Played a role in development of a number of assays including viral genome quantification by qPCR, host cell protein quantification by ELISA and endotoxin level testing by LAL chromogenic assay
- DNA extraction, DNA sequencing sample preparation and data analysis using snapgene
- Tissue culture-mammalian cells maintenance at GMP facility
- Played a major role in the development of pseudo viruses for gene therapy
- Generate quality data and study summary reports in a timely manner and communicate
- Author protocols, reports, SOPs to document developmental studies and ensure method standardization
- Compile data/figures and generate comprehensive presentations to communicate results and plans with key stakeholders
- Streamlined research processes to meet tight deadlines for multiple projects.
- Enhanced research efficiency by optimizing experimental designs and protocols.
Teaching and leadership
University of Manitoba
05.2017 - 12.2021
- Trained many master's graduate students starting with cell culture maintenance, protein production, purification, and protein quality evaluation
- Mentored and supervised undergraduate students, guiding them towards academic success and personal growth.
Teaching Assistant Biochemistry
University of Manitoba
01.2016 - 01.2021
- Coordinated and graded 40+ undergraduate students' lab, and designed entry level biochemistry experiments
- Graded lab reports and provided detailed written feedback weekly
Lecturer
Mahindra Educational Pvt. Ltd.
01.2013 - 01.2015
- Evaluated a class of 50 students for Biology and Chemistry (competitive examinations)
- Supported in developing study material, multiple choice questions, and mock tests
- Conducted discussion sessions for students to make explanations easy for Arts students resulting in strengthening communication and presentation skills
Education
Ph.D. - Biochemistry
University of Manitoba
07.2022
Master of Science - Human Genomics
Panjab University
05.2012
Bachelor of Education -
BAR Janta College
India05.2014
Bachelor of Science -
Panjab University
05.2010
French Language (B1 Level) -
Alliance Francaise
08.2014
Skills
- Laboratory techniques
- Safety processes and procedures
- Assay development
- Research methods
- Protein Purification
- Experimental design
- Quantitative skills
- Research and publication
- Cell Culture Techniques
- Immunological Assays
- Spectroscopy Techniques
- Project Management
- Chromatography
- Spectroscopy Techniques
- Computational modeling
- Effective Multitasking
- Critical Thinking
- Chromatography
- Data Analysis
- Scientific Writing
Accomplishments
- Hugh J. Anderson Graduate Award in Chemistry, University of Manitoba, 01/2020
- University of Manitoba Graduate Scholarship, 05/2018 - 04/2020
- McCrorie-West Family Fellowship for Alzheimer Research, Fall 2017 - Winter 2018 and Fall 2018 - Winter 2019
- Hugh J. Anderson Graduate Award in Chemistry, University of Manitoba, 01/2017
- Manitoba Graduate Scholarship, 05/2016 - 04/2018
- International Graduate Student Entrance Scholarship, 09/2016 - 04/2017
- Gold medalist (first rank holder) in M.Sc. Human Genomics, India
Research
- Ph.D., Biochemistry, Department of Chemistry, University of Manitoba, CANADA, 09/2016, 05/2022, Studies on receptor-ligand interactions (Netrin-Deleted in Colorectal Cancer/Neogenin/Unc5)
- Research Assistant, Department cum National Centre for Human genome studies, Panjab University, INDIA, 01/2010, 12/2012, Studies on localization of SRD5A2 gene product in prostate cancer cell lines using GFP-SRD5A2 reporter construct
- Summer Researcher, Biochemistry and Molecular Biology Lab, Centre of Advanced Study, Banaras Hindu University, INDIA, 06/2011, 08/2011, Hands-on practice on cell culture and maintenance of several cell lines, isolation and characterization of RNA from mouse tissues, RT-PCR, Dot-blotting, and EMSA.
Publications
The repulsive power of Unc5-Molecular insights into the netrin-1 Unc5 complex formation., to be submitted,
Investigation of dynamic solution interactions between NET-1 and UNC-5B by multi-wavelength analytical ultracentrifugation., European Biophysics, 2023,
The dynamic nature of netrin-1 and the structural basis for glycosaminoglycan fragment-induced filament formation., Nature communication, 14, 1226, 2023,
Heparins mediate the multimer assembly of extracellular Noggin., Protein Science, 31, 2022,
Homogeneous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor., Applied Microbiology and Biotechnology, 105, 6047-6057, 2021,
Microfluidic Devices for studying the effect of Netrin-1 on Neutrophil and Breast Cancer Cell Migration., Adv. Biosyst., 5, 1700178, 2018,
Timeline
Scientist
Novel Biotechnology Inc.
07.2023 - Current
Research Scientist
Genvira Biosciences
10.2022 - 04.2023
Teaching and leadership
University of Manitoba
05.2017 - 12.2021
Teaching Assistant Biochemistry
University of Manitoba
01.2016 - 01.2021
Lecturer
Mahindra Educational Pvt. Ltd.
01.2013 - 01.2015
Ph.D. - Biochemistry
University of Manitoba
Master of Science - Human Genomics
Panjab University
Bachelor of Education -
BAR Janta College
Bachelor of Science -
Panjab University
French Language (B1 Level) -
Alliance Francaise
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