Melissa Izaguirre

• Studied E.coli during Long Term Stationary Phase (LTSP) in a lab setting
• Prepare Escherichia coli for lysis and transductions to compete against wild-type Escherichia coli
• Run colony PCR and gel electrophoresis
• Keep a lab notebook with procedures and findings
• Present findings though a poster presentation during conferences
• Maintaining an organized lab bench for experiments
• Performed Datsenko-Wanner Chromosomal Gene Knockouts on e.Coli
• Analyzed results and created reports
• Prepared reagents and solutions following standard laboratory formulas and procedures
• Collaborated with peers to design and execute experiments
• Provided support and guidance to other lab staff
• Adhered to laboratory safety procedures
• Used multichannel pipettes to titer samples
• Performed research into study topics to better understand what was being worked with

• Contributed to lesson planning and course/assignment design
• Supervision of 19 students during class and recreation
• Participate and lead class/study hours, academic enrichment activities, and scholar community time
• Work with other 4 other staff members
• Drove scholars to and from activities/internships
• Facilitate class activities
• Participated in daily staff meeting with updates on curriculum and student updates/concerns

• Supervised and guided 40 students during class time
• Lesson planning
• Design weekly lab experiments relevant to the class topic
• Facilitate class activities
• Introducing scientific thinking to the students

Additional work dates:

June 2019 - July 2019
May 2021 - July 2021

• Cultured E.coli and other pathogenic bacteria
• Operated PCR machine (thermocycler), gel electrophoresis, western blots, autoclave, fumehoods, centrifuges, and other lab equipment
• Presented research findings
• Organized and summarized findings in a lab notebook
• Restocked lab with necessary regents

• Handled rats and assisted in maintaining operant chambers sterile
• Injected antibiotics into rats after their exposure to alcohol or cocaine
• Inserted data on rat reactions into an Excel sheet for a report
• Present research findings to peers and instructors