Marwa Dlim

To improve my research skills and heighten my knowledge in the pharmaceutical and medical fields. HPLC NMR (Nuclear Magnetic Resonance). Cell Culture Based Assay. DLS (Dynamic Light Scattering). TEM (Transmission electron micrograph) Confocal Microscopy. Student-centered Inspiring Professor brings advanced teaching skills and department leadership experience. Goal-oriented to advance department, improve teaching and modernize courses. Extensive background in research and university fundraising. Organized and dependable candidate successful at managing multiple priorities with a positive attitude. Willingness to take on added responsibilities to meet team goals.

• Assessing Compound Self-aggregation in Drug Discovery and Development
• In aqueous solution small molecules exist in a unique three-phase equilibrium between single lone- tumbling molecules, self-associated aggregates, and solid forms
• The nature of the equilibria profoundly dictates compound properties; however, characterization of this phenomenon remains relatively unexplored, particularly from a pharmaceutical perspective
• For instance, employing dynamic light scattering (DLS) has determined that some molecules can self-assemble into micelle- like colloids and bind proteins, resulting in false-positive results in drug screens
• However, the existence of smaller aggregates or nano-entities have historically been undetectable and thus their corresponding properties remain uncharacterized
• Here, we present methodologies for monitoring the molecular properties of compound aggregates
• First, we employ nuclear magnetic resonance (NMR) spectroscopy as a practical and valuable tool to directly observe lone molecules, small nano- entities, and indirectly, by observing large micelle-like aggregates
• In this study, we also employ
• Marwa M
• Dlim C.V, DLS for characterizing large molecular aggregates, and examine the self-association phenomena between dyes and drugs using transmission electron microscopy (TEM)
• This method clearly exposes large micelle-like particles of variable size
• We employ the long-held assumption that molecular aggregates cannot form within cells
• We discovered that dyes and drugs can form aggregates intracellularly in HeLa and Huh-7 cells, having discrete nuclear or cytoplasmic localization
• As determined by confocal laser scanning microscopy (CLSM)
• Implementing the above strategies expose a wide variety of molecular compound behaviors in solution and within cells
• Given this new set of tools, screens can be implemented to aid in the understanding of the physical properties of compound aggregates such as, toxicity, off-target activity and loss of efficacy
• These molecular properties provide new insights on how molecules behave, in vitro and in vivo, which can be exploited for drug new properties such as encapsulation and delivery systems
• Award and Fellowships
• A governmental scholarship for studying full time Master degree in Canada
• Http://www.uot.edu.ly/
• Professor
• Steven LaPlante
• Medicinal chemistry, Pharmacological innovation
• Scientific co-head of the Nuclear
• Magnetic Resonance Spectroscopy

Publications, Communications, & Participation in International Meetings & Workshops

1. Nagib Ali Elmarzugi, Eseldin I. Keleb, Aref T. Mohamed, Hesham A. El Enshasy, Amal M. Hamza, Marwa M. Dlim, Ahmed A. Layla, Mohamed Salama (2013), Face Powder Problems Perception Survey, International Journal of Pharmaceutical Science Invention, Volume 2, Issue 6, June, PP.09-18.
2. Marwa M. Dlim, Sami G. Alsabri, Abdulmottaleb E. Zetrini, Sofian S. Mohamed, Asma A.H. Salem, and Abdurazag A. Auzi (2013) Use of Beta vulgaris as Natural Coloring Agent for Foods and Cosmetics in Libya, Journal of Chemical and Pharmaceutical Research,
5(11):340-45.
3. Murugesan, J. R., Shahout, F., Dlim, M., Langella, M. M., Cuadra-Foy, E., Forgione, P., & LaPlante, S. R. (2018). Revealing dye and dye-drug aggregation into nano-entities using NMR. Dyes and Pigments, 153, 300-306. doi:10.1016/j.dyepig.2018.02.026.
4. Food Safety Management workshop, under World Food Safety Organization, May
2012, Turkey.
5. 4. Principles of Clinical Ultrasound in diagnosis, Training program, Oct, 2011, In Vitro Fertilization, Gynecology and Obstetrics Clinic (Ghadmour Clinic), Alhadba Alkhadra, Tripoli, Libya.
6. The first Turkish-international Circle’s workshop on food science and technology, 9-11 May
2013, Kayseri, Turkey.
7. 8th International Scientific Symposium "Ethical and moral of the professional of pharmacy" (2010) organized by Libyan Society for Pharmaceutical Sciences, 28 June, Tripoli-Libya.
8. Marwa M Dlim 1, Fatma S Shahout 1, Marwa K Khabir 1, Patrick P Labonté 1, Steven R LaPlante (2019) Revealing Drug Self-Associations into Nano-Entities