Malini Subramaniam
San Carlos
Summary
Determined and well-rounded individual with more than 5 years working as Scientist within cell & gene therapy industry. Sharp and focused professional offering skills in cell engineering, stem cell research, microbiology and data analysis. Pursuing role and leveraging exceptional technical knowledge to advance therapies for cancer and autoimmune diseases.
Overview
5
5
years of professional experience
Work History
Scientist
Allogene Therapeutics
08.2022 - 01.2024
- Enhanced research efficiency by evaluating CAR-T construct designs using programmable nuclease and site-specific integration of transgene by viral and non-viral delivery systems (LV, AAV, CRISPR).
- Designed assays to assess the functionality, specificity and safety of CAR-T cells using expertise in molecular biology techniques, yielding robust results that supported key hypotheses.
- Performed preliminary and exploratory small molecule screening studies to improve CAR-T phenotype.
- Using proficiency in Flow-Jo and GraphPad Prism software, documented and reported test results after making graphical solutions and representations.
Senior Research Associate
CRISPR Therapeutics
12.2019 - 08.2022
- Advanced company pipeline for Type-I diabetes treatment by developing and characterizing pancreatic islet beta cells from stem cells, using robust 3D organoid differentiation protocols.
- Optimized cost-effective experimental procedures to develop CAR-NK cells from iPSCs to treat solid tumors, while maintaining accuracy and reliability of results.
- Initiated, planned and executed advance functional studies using molecular biology techniques to assess functionality, safety and phenotype of cell products. (flow cytometry, in-vitro cytotoxicity assays)
- Gene editing of iPSCs using CRISPR-Cas9 and generation of clonal populations
- Managed multiple projects simultaneously, ensuring timely completion and high-quality deliverables.
- Led efforts to optimize and develop new assays for better functional readouts that would predict success in in-vivo studies.
- Proactively volunteered for lab management duties and leveraged highly effective communication, active listening and organization skills to collaborate with scientists.
Scientist I
PBL Assay Science
10.2018 - 11.2019
- Planned and executed custom immunoassays - ELISA, MSD and bead-based assays (Simoa / Singulex) for high-throughput screening of clinical samples.
- Performed mammalian cell culture to use in cell proliferation & antiviral assays.
- Purified antibody fragments from whole IgG using affinity chromatography.
- Established partnerships with academic institutions for supporting research initiatives and resource sharing opportunities.
- Maintained accurate records of all methods and research conducted and wrote in-depth research reports for clients.
- Managed laboratory operations, including purchasing lab supplies, troubleshooting of equipment and maintaining stock inventories.
Education
Master of Science - Biomedical Science
Rutgers, The State University of New Jersey
Newark, NJ08.2018
Bachelor of Technology - Biotechnology
Vellore Institute of Technology, India
Vellore, India05.2016
Skills
- Gene Editing (CRISPR, AAV, LV)
- Stem cell differentiation
- Aseptic cell culture (primary and mammalian cells)
- Assay Development - cytotoxicity assay, ELISA
- Multi-color flow cytometry (up to 14 colors)
- RT-PCR
- NGS library preparation
- Western blotting
- Histology (IHC, immunofluorescence)
Publications
D'Mello, Veera et al. “Intranasal Leukemia Inhibitory Factor Attenuates Gliosis and Axonal Injury and Improves Sensorimotor Function After a Mild Pediatric Traumatic Brain Injury.” Neurotrauma reports vol. 4,1 236-250. 11 Apr. 2023, doi:10.1089/neur.2021.0075
Websites
Timeline
Scientist
Allogene Therapeutics
08.2022 - 01.2024
Senior Research Associate
CRISPR Therapeutics
12.2019 - 08.2022
Scientist I
PBL Assay Science
10.2018 - 11.2019
Master of Science - Biomedical Science
Rutgers, The State University of New Jersey
Bachelor of Technology - Biotechnology
Vellore Institute of Technology, India
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