Practical Research, Designed and manufactured of home-made Fluorescent Structured Illumination Microscope(SIM), Shahid Beheshti University, Laser and Plasma Research Institute (LAPRI), Tehran, Tehran, 01/01/17, Present, The Point Spread Function (PSF) is measured through an experimental and analytical method (Maximum Likelihood Estimation algorithm (MLE)) using MATLAB software., Improved resolution of images by using measured PSF, structured illumination microscope and SIM reconstruction algorithm in in-vitro imaging. Computer Simulation, Designed and simulated a Fluorescent Structured Illumination Microscope in Zemax, Shahid Beheshti University, Laser and Plasma Research Institute (LAPRI), Tehran, Tehran, 09/01/17, 02/01/19 Specimen Preparation, Tissue preparation for Light Microscopy of Rat brain, Tissue Fixation, Embedding and Sectioning, Fresh freezing and cry sectioning, Tissue Staining using fluorescence and non-fluorescence dyes, Cell Culture Immunofluorescence, Neuroscience Research Center(NRC), Shahid Beheshti University, Tehran, Tehran, 09/01/17, Present Cancer Cell Detection, Detection of cancer cells according to size and color using LabView Software, Shahid Beheshti University, Tehran, Tehran, 09/01/17, 12/01/17 Creation Pulse Oximeter, Measurement blood oxygen using Arduino and LabView, Shahid Beheshti University, Tehran, Tehran, 09/01/17, 12/01/17