Performing RT-qPCR and Sandwich ELISA assays, followed by a critical analysis of the results
MIMM384: Laboratory Molecular Biology
Characterizing engineered plasmids through restriction analysis, PCR amplification and gel electrophoresis visualization
Extracting and purifying a genetically tagged protein of interest from cell lysate using pull-down assays
Quantifying protein expression with western blotting
Assessing bacteriophage virulence and selecting bacteriophage-insensitive (BIM) bacterial mutants using plaque assays
Studying Bacteria-Bacteriophage co-evolution: analyzing BIM spacer acquisition with CRISPR-Cas loci PCR amplification, and characterizing mutations in virulent bacteriophage from nanopore sequencing genomic data
MIMM212: Laboratory Microbiology
Identifying the strain of antibiotic-producing, soil-derived bacterial isolates, critically comparing nanopore DNA sequencing results to BLAST database matches
Determining the bacterial antibiotic resistance profile through spread and patch assays
Gram-staining bacterial specimens for microscopic examination
Isolating and culturing BCL2 microorganisms
MIMM301: Scientific writing skills
Synthesizing primary research into a literature review