Kai Sheng
Dorval,Québec
Summary
Biotech researcher with 10+ years of experience in molecular and cellular biology, preclinical models, and translational research. Skilled in assay development, RNA-seq analysis, and collaboration with clinicians and researchers in multidisciplinary teams. Proven track record of publications, scientific presentations, and award-winning research focused on cartilage, intervertebral disc, and bone biology, with emphasis on sterile inflammatory responses and aging-related degeneration.
Overview
10
10
years of professional experience
Work History
Surgical Assistant (On-call)
Transplant Quebec
Montreal, QC
01.2020 - Current
- Assisted orthopedic fellows in harvesting 50+ human spine and tissue samples in an operating room setting, ensuring compliance with clinical protocols.
- Coordinated intraoperative sample handling, X-ray imaging, and secure data management.
- Recorded disc conditions, pain questionnaires, and clinical data using REDCap and spreadsheets for research integration.
Medical Biologist
Shriners Hospitals for Children
Montreal, QC
01.2018 - 01.2021
- Conducted molecular and cellular assays (RNA-seq, qPCR, IHC, CRISPR KO) to study bone/cartilage biology and inflammatory pathways.
- Collaborated with clinicians and research fellows; managed lab operations and contributed to multi-author publications.
Founder
KS Production
Montreal, QC
01.2017 - 01.2021
- Provided professional scientific photography for 200+ trainees and medical professionals.
- Documented academic conferences and developed outreach materials.
Research Assistant
McGill University Health Centre
Montreal, QC
01.2016 - 01.2018
- Conducted genetic research on HIV and tuberculosis, including mouse tissue dissection, RNA extraction, library preparation for sequencing, and PBMC isolation from patient and donor blood.
- Performed molecular assays (ELISA, Western blot) and microscopy for cellular and protein analysis.
- Contributed to manuscripts and conference abstracts.
Education
Ph.D. - Experimental Surgery
McGill University
Canada08.2025
Gr.Dip - Clinical Research
McGill University
Canada01.2021
M.Sc. - Experimental Medicine (Thesis)
McGill University
Canada01.2016
B.Sc. - Biotechnology
Sichuan Normal University
China01.2011
Skills
Molecular Biology: RNA/DNA extraction, qPCR, ELISA, Western blot, IHC, CHIP, RNAseq
Cell Biology: Cell culture, CRISPR KO, RNAi, overexpression, luciferase assays
Animal Models: Mouse dissection, microCT analysis, metabolic assays (GTT, ITT)
Clinical/OR: Human tissue procurement, PBMC isolation, intraoperative sample management, clinical data recording
Software: GraphPad Prism, R studio, ImageJ/QuPath, Adobe Illustrator, Photoshop,
Selected Achievements
- Awarded Doctoral Training Scholarship (FRQS, 2023-2026) - ranked 1st in competition group.
- Recipient of multiple travel awards and research presentation prizes (2022-2024).
- First/co-first author publications in Arthritis Research & Therapy, BMC Gastroenterology, Osteoarthritis & Cartilage, Cells, and others.
- Oral/poster presentations at international conferences (ORS, CCTC, ISGD).
Accomplishments
• Awarded Doctoral Training Scholarship (FRQS, 2023–2026) – ranked 1st in competition group.
• Recipient of multiple travel awards and research presentation prizes (2022–2024).
• First/co-first author publications in Arthritis Research & Therapy, BMC Gastroenterology, Osteoarthritis & Cartilage,
Cells, and others.
• Oral/poster presentations at international conferences (ORS, CCTC, ISGD).
Timeline
Surgical Assistant (On-call)
Transplant Quebec
01.2020 - Current
Medical Biologist
Shriners Hospitals for Children
01.2018 - 01.2021
Founder
KS Production
01.2017 - 01.2021
Research Assistant
McGill University Health Centre
01.2016 - 01.2018
Ph.D. - Experimental Surgery
McGill University
Gr.Dip - Clinical Research
McGill University
M.Sc. - Experimental Medicine (Thesis)
McGill University
B.Sc. - Biotechnology
Sichuan Normal University
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