Microsoft Office
Hedieh Sattarifard
Winnipeg,MB
Summary
In my background, I specialize in researching post-translational modifications on histones, focusing primarily on leukemia, particularly mixed lineage leukemia. Additionally, I have expertise in studying genetic polymorphisms associated with long non-coding RNA and microRNA in breast and prostate cancer
Overview
2
2
years of professional experience
1
1
Language
Work History
Molecular Biology Lab Technician
Zahedan University Of Medical Sciences
12.2018 - 12.2020
- Perform laboratory experiments and tests using molecular biology techniques, including PCR, DNA sequencing, and gel electrophoresis.
- Design primers tailored for gene expression studies and Chromatin Immunoprecipitation (ChIP) experiments, ensuring precise targeting of specific genomic regions and accurate analysis of molecular interactions.
- Operate and maintain laboratory equipment such as thermal cyclers, electrophoresis systems, and spectrophotometers.
- Draft and refine Standard Operating Procedures (SOPs) to establish rigorous protocols, maintain consistency in laboratory methodologies, and adhere to regulatory standards.
- Contribute to the preparation of scientific reports, manuscripts, and presentations for internal use or publication in peer-reviewed journals, including data analysis and literature review.
- Collaborate with research scientists, principal investigators, and other laboratory staff to plan and execute experiments, troubleshoot technical issues, and interpret result
Education
Master of Science - Biochemistry And Medical Genetics- Epigenetics
University of Manitoba
Winnipeg, MB 05.2001 -
Master of Science - Clinical Biochemistry
Zahedan University of Medical Sciences
Zahedan, Iran 05.2001 -
Bachelor of Science - Cell And Molecular Biology- Biochemistry
Islamic Azad University of Mashhad
Mashhad, Iran 05.2001 -
Skills
Accomplishments
Publications:
1. Bioinformatic Analyses of Broad H3K79me2 Domains in Different Leukemia Cell Line Data Sets
Prerna Sharma, Hedieh Sattarifard, Narges Fatemiyan, Ted M Lakowski, James R Davie Cells 2022, 11(18), 2830; https://doi.org/10.3390/cells11182830
2. Mitogen- and stress-activated protein kinase (MSK1/2) regulated gene expression in normal and disease states
Hedieh Sattarifard, Akram Safaei, Enzhe Khazeeva, Mojgan Rastegar, James R Davie Biochemistry and Cell Biology. 2023/2/22 https://doi.org/10.1139/bcb-2022-0371
3. Association between genetic polymorphisms of long noncoding RNA PRNCR1 and prostate cancer risk in a sample of the Iranian population
Sattarifard H, Hashemi M, Hassanzarei S, Narouie B, Bahari G.
Mol Clin Oncol. 2017 Dec;7(6):1152-1158. doi:10.3892/mco.2017.1462.
4. Long noncoding RNA POLR2E gene polymorphisms increased the risk of prostate cancer in a sample of the Iranian population
H Sattarifard, M Hashemi, S Hassanzarei, A Basiri, B Narouie, S Ghavami
Nucleosides Nucleotides Nucleic Acids. 2019;38(1):1-11. doi: 10.1080/15257770.2017.1391394.
5. Genetic polymorphisms of HOTAIR gene are associated with the risk of breast cancer in a sample of southeast Iranian population
Shekoufeh Hassanzarei, Mohammad Hashemi, Hedieh Sattarifard, Seyed Mehdi Hashemi, Gholamreza Bahari, Saeid Ghavami
Tumour Biol. 2017 Oct;39(10):1010428317727539. doi: 10.1177/1010428317727539.
6. Genetic polymorphisms in long noncoding RNA H19 are associated with breast cancer susceptibility in Iranian population
Shekoufeh Hassanzarei, Mohammad Hashemi, Hedieh Sattarifard, Seyed Mehdi Hashemi, Gholamreza Bahari
7. Evaluating the Possible Association between PD-1 (Rs11568821, Rs2227981, Rs2227982) and PD-L1 (Rs4143815, Rs2890658) Polymorphisms and Susceptibility to Breast Cancer in a Sample of Southeast Iranian Women
Karami S, Sattarifard H, Kiumarsi M, Sarabandi S, Taheri M, Hashemi M, Bahari G, Ghavami S.
Asian Pac J Cancer Prev. 2020 Oct 1;21(10):3115-3123. doi: 10.31557/APJCP.2020.21.10.3115.
8.Association between genetic polymorphisms of miR-1307, miR-1269, miR-3117 and breast cancer risk in a sample of south east Iranian women
Sarabandi S, Sattarifard H, Kiumarsi M, Karami S, Taheri M, Hashemi M, Bahari G, Ghavami S.
Asian Pac J Cancer Prev. 2021 Jan 1;22(1):201-208. doi: 10.31557/APJCP.2021.22.1.201.
9. Detection of a 4-bp insertion/deletion polymorphism within the promoter of EGLN2 using mismatch PCR-RFLP and its association with susceptibility to breast cancer
Hashemi M, Danesh H, Bizhani F, Sattarifard H, Hashemi SM, Bahari G.
Asian Pac J Cancer Prev. 2018 Apr 25;19(4):923-926. doi: 10.22034/APJCP.2018.19.4.923
10. Toxicity, therapeutic applicability, and safe handling of magnetic nanomaterials
Yasser Al-Eryani, Maryam Dadashi, Sajjad Aftabi, Hedieh Sattarifard, Ghazal Ghavami, Zachary William Oldham, Arash Ghoorchian, Saeid Ghavami
Magnetic Nanomaterials in Analytical Chemistry .2021/1/1. https://doi.org/10.1016/B978-0-12-822131-0.00012-1
11. Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism
Hashemi M, Bahari G, Sattarifard H, Narouie B.
Mol Clin Oncol. 2017 Oct;7(4):696-700. doi:10.3892/mco.2017.1369.
Award
Awarded the Human Genetics Endowment Fund Graduate Student Award for the year 2023 at the University of Manitoba
Volunteer Experience
CMSB Committee Member [2023 – Present]
University of Manitoba
- Actively contribute as a committee member for the CMSB (Cellular and Molecular Biology) organization. Collaborate with fellow committee members to plan, organize, and execute events and activities.
Software
UCSC Genome Browser
NCBI Blast
Affiliations
Max Rady College of Medicine, Biochemistry and Medical Genetics Department, Disstingused Professor James Davie Lab
Additional Information
References:
- James Davie, PhD, FCAHS, FRSC, Distinguished professor
Max Rady College of Medicine, Biochemistry and Medical Genetics
Room 333A – Basic Medical Sciences Building
745 Bannatyne Avenue
University of Manitoba
Winnipeg, Manitoba R3E 0J9
Phone: 204-272-3174
jim.davie@umanitoba.ca
- Ted Lakowski, PhD, Associate professor
College of Pharmacy, Apotex Centre, 750 McDermot Ave. W. University of Manitoba, Winnipeg, MB R3E 0T5
Phone:204-272-3173
ted.lakowski@umanitoba.ca
Timeline
Molecular Biology Lab Technician
Zahedan University Of Medical Sciences
12.2018 - 12.2020
Master of Science - Biochemistry And Medical Genetics- Epigenetics
University of Manitoba
05.2001 -
Master of Science - Clinical Biochemistry
Zahedan University of Medical Sciences
05.2001 -
Bachelor of Science - Cell And Molecular Biology- Biochemistry
Islamic Azad University of Mashhad
05.2001 -
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