Harinee Srikannan
Richmond,BC
Summary
Dynamic research technician and lab manager at the Lady Davis Institute for Medical Research, skilled in molecular biology techniques and data analysis. Achieved over 95% experimental reproducibility while training teams and optimizing workflows. Proven ability in managing laboratory operations and ensuring compliance, with a strong focus on collaboration and effective communication.
Overview
2
2
years of professional experience
Work History
Research technician & Lab Manager
Lady Davis Institute for Medical Research
Montreal, Quebec
01.2024 - Current
- Performed a wide range of molecular biology techniques (qPCR, PCR, gene silencing, bacterial transfection) across more than 10 oncology projects, achieving greater than 95% experimental reproducibility.
- Executed comprehensive histology and tissue imaging workflows, including tissue processing, microtome, IHC, IF, slide preparation, and CODEX; prepared over 1,000 slides, and conducted quantitative analysis for more than 300 human and murine samples.
- Conducted bacterial transfection and functional assays with 80–90% efficiency, supporting studies on gene regulation, signaling pathways, and tumor biology.
- Maintained four mammalian cell lines, prepared more than 50 reagents and solutions, and supported continuous workflow across multiple research projects.
- Supported animal handling procedures (injections, weaning, monitoring) for 500–2,000 mice annually, ensuring full compliance with the McGill Animal Care and Use Program and CCAC guidelines.
- Trained new staff, students, and visiting researchers on molecular assays, histology workflows, imaging techniques, and lab safety practices.
- Integrated high-throughput sequencing data with microscopy and immunofluorescence imaging to investigate tumor progression, gene expression, and therapeutic response.
- Collaborated with multidisciplinary teams on experimental design, troubleshooting, data analysis, and prepared figures, reports, manuscripts, and grant submissions.
- Managed day-to-day operations for a 15-member laboratory, including workflow coordination, equipment use, troubleshooting, and onboarding new personnel.
- Oversaw laboratory inventory and procurement for over 500 consumables annually, maintained accurate supply logs, negotiated vendor quotes, and reduced ordering delays by 30%.
- Developed, edited, and submitted Animal Use Protocols (AUPs) through McGill’s ethics system, including detailed animal number justification for 500 to 2,000 mice in multi-phase cancer studies.
- Prepared documentation for internal safety inspections and ethics audits, ensuring zero noncompliance findings.
- Standardized and optimized experimental protocols and lab workflows, improving reproducibility by 20–30%, and reducing processing time by 15%.
Research Intern
Lady davis Institute for Medical research
Montreal, Quebec
05.2023 - 12.2023
- Investigated arsenic- and cadmium-induced toxicity using in vivo mouse models and in vitro cell-based assays, contributing to a mechanistic understanding of metal toxicity.
- Performed RNA and DNA extractions, qPCR, and immunohistochemistry (IHC) for gene expression and tissue-level analyses, generating high-quality, reproducible datasets.
- Assisted with mouse handling, tissue collection, and sample processing, ensuring full compliance with institutional and CCAC animal care protocols.
- Compiled and analyzed toxicological data sets, supported experimental conclusions, and prepared summary reports for collaborative group discussions and lab meetings.
- Contributed to collaborative research efforts that resulted in authorship on a peer-reviewed publication (2024, accepted/in press).
Education
Master of Science - Applied Biotechnology
McGill University
Montreal, Quebec12-2023
Bachelor of Technology - Biotechnology
Sri Venkateswara College of Engineering
Chennai, India08-2022
Skills
- PCR and QPCR
- Bacterial transformation
- Gene silencing
- Protein purification
- Cytotoxicity assays
- Tissue processing and embedding
- Immunohistochemistry (IHC)
- Immunofluorescence (IF)
- CODEX multiplexed tissue imaging
- Mouse handling and dissection
- Laboratory inventory management
- Experiment planning and coordination
- Quality control and compliance
- Ethical documentation and safety audits
- Team training and leadership
- Data analysis and scientific reporting
- Microsoft Office Suite proficiency
- 3D printing and TinkerCAD skills
- Effective communication and collaboration
- Attention to detail and time management
Leadership Experience
- Managed day-to-day operations for a 15-member laboratory, coordinating workflows, equipment use, troubleshooting, and onboarding new personnel
- Played the role of Treasurer, Society of Biotechnology Forum (SBF), Organized global webinars with scientists and researchers. Coordinated core team activities and managed event logistics.
Languages
English
Native/ Bilingual
French
Limited
Timeline
Research technician & Lab Manager
Lady Davis Institute for Medical Research
01.2024 - Current
Research Intern
Lady davis Institute for Medical research
05.2023 - 12.2023
Master of Science - Applied Biotechnology
McGill University
Bachelor of Technology - Biotechnology
Sri Venkateswara College of Engineering