Hanan Babeker
Post-Doctoral Fellow In Antibody Engineering And Nuclear Medicine
Saskatoon,SK
Overview
7
7
years of professional experience
Work History
Post-Doctoral Fellow
University Of Saskatchewan
09.2023 - Current
- Developed antibody based theranostics for PET imaging and radioimmunotherapy of ovarian, pancreatic, and breast cancer.
- Developed fully human antibodies using synthetic antibody libraries by phage display.
- Applied multiple innovative selection techniques to synthetic and immune antibody libraries, like phage display, FACS sorting, plate-based screening.
- Sub-clone hits into expression vectors and produce them at micro-scale in a high-throughput manner.
- Engineer and improve binders by means of affinity maturation or humanization.
- Antibody expression in EXPi293 mammalian cells and purification using affinity chromatography.
- Characterized antibody for binding affinity and specificity using SPR and flow cytometry, internalization and in-vitro cytotoxicity using InCucyte.
- Developed antibody based PET/SPECT imaging probe using different radioisotopes.
- Developed antibody based radiopharmaceuticals using alpha and beta radioisotopes.
- Experience with a range of in vitro skills, e.g. cell culture, molecular biology, immunoblotting, flow cytometry and ELISA.
- Experience developing and working with complex in vivo models, such as xenograft, patient derived xenograft (PDX), syngeneic mouse models.
- Experience in tumor inoculation, measurement, SC, IV dosing, blood, and tissue collection.
- Experience delivering in vivo pharmacology studies in mouse tumor models, including biodistribution, dose-finding tolerability studies, PK and efficacy studies.
- Experience designing, conducting, and analyzing combination therapies for cancer including antibody-drug conjugates (ADCs), antibody-radioimmuno conjugates (RIC) in combination with immune check-point inhibitors (ICI).
- Generate hypotheses to test, troubleshoot issues, design and execute the next set of experiments with the input from team members and supervisor.
- Document the results in a detailed manner in laboratory journal and in internal databases.
- Present research updates and participate constructively in working project teams and at laboratory group meetings.
- Authored professional scientific papers for publishing in peer-reviewed journals.
- Troubleshot and developed experimental tools to deal with ever-evolving technology.
- Collaborated with colleagues to gather research and publish findings.
- Attended research seminars and conferences to discover and discuss new technologies and research.
Quality Assurance Officer - Clinical Trial
University Of Saskatchewan
04.2023 - Current
- Ensuring all processes are controlled and monitored.
- processing quality audits and quality assurance reviews.
- Documenting new and existing processes.
- Monitored activities and supporting systems to meet compliance regulations.
Teaching Assistant
University Of Saskatchewan
09.2019 - 08.2023
- Assisted teachers with classroom management and document coordination to maintain positive learning environment.
- Helped with grading assignments and tests, providing constructive feedback to students based on results.
- Handled class records for attendance, assignment grades and course participation scores.
Research Assistant
University Of Saskatchewan
05.2017 - 09.2017
- Developed small protein based affinity binders by phage display technology.
- Performed molecular biology techniques like tissue culture, cloning, PCR, protein expression and purification using affinity chromatography, protein binding ELISA assay, and flow cytometry.
Education
Ph.D. - Health Sciences
University of Saskatchewan
Saskatoon, SK, Canada09.2023
Bachelor of Science - Biochemistry
University of Saskatchewan
Saskatoon, SK, Canada06.2017
Publications and Presentations
Publications
- Babeker, H.; Ketchemen, J.P.; Annan Sudarsan, A.; Andrahennadi, S.; Tikum, A.F.; Nambisan, A.K.; Fonge, H.; Uppalapati, M. Engineering of a Fully Human Anti-MUC-16 Antibody and Evaluation as a PET Imaging Agent. Pharmaceutics 2022, 14, 2824. https://doi.org/10.3390/pharmaceutics14122824.
- Ketchemen, J.P., Babeker, H., Tikum, A.F. et al. Biparatopic anti-HER2 drug radioconjugates as breast cancer theranostics. Br J Cancer 129, 153–162 (2023). https://doi.org/10.1038/s41416-023-02272-4.
- Tikum, A.F.; Nambisan, A.K.; Ketchemen, J.P.; Babeker, H.; Khan, M.N.; Torlakovic, E.E.; Fonge, H. Simultaneous Imaging and Therapy Using Epitope-Specific Anti-Epidermal Growth Factor Receptor (EGFR) Antibody Conjugates. Pharmaceutics 2022, 14, 1917. https://doi.org/10.3390/pharmaceutics14091917.
- McDonald S, Annan Sudarsan A, Babeker H, Budharaju K, Uppalapati M. Generation of Protein Inhibitors for Validation of Cancer Drug Targets Identified in Functional Genomic Screens. Methods Mol Biol. 2021;2381:307-331. doi: 10.1007/978-1-0716-1740-3_17. PMID: 34590284.
- Add three papers already accepted.
Posters and Presentations
- 225Ac/89Zr-labeled anti-nectin-4 radioimmunoconjugates as theranostics against nectin-4 positive triple negative breast cancer. American Association for Cancer Research (AACR) Conference, San Diego, USA (2024).
- Development and initial evaluation of a fully human anti-nectin-4 antibody as theranostic probes for nectin-4 positive triple negative breast cancers. Society of Nuclear Medicine and Molecular Imaging SNMMI Conference, Vancouver, Canada (2022).
- Development of theranostic probes targeting ovarian cancer biomarker MUC16/CA125. 4th Protein Engineering Conference Canada, Montreal, Canada (2022).
- Development of small protein based affinity reagents targeting ovarian cancer biomarker MUC16 (CA125). Next Generation Protein Therapeutics Summit, San Francisco, USA (2019).
- Biparatopic anti-HER2 drug radioconjugates as breast cancer theranostics. Society of Nuclear Medicine and Molecular Imaging SNMMI Conference, Vancouver, Canada (2022).
- Simultaneous Imaging and Therapy Using Epitope-Specific Anti-Epidermal Growth Factor Receptor (EGFR) Antibody Conjugates. Society of Nuclear Medicine and Molecular Imaging SNMMI Conference, Vancouver, Canada (2022).
- Development of small protein based affinity reagents targeting MUC16 or CA125. Protein Structure, Function and Malfunction (PSFaM) conference, Saskatoon, Canada (2019).
- Development of small protein based affinity reagents targeting MUC16 or CA125. Saskatchewan Cancer Research Conference (SCRC), Saskatoon, Canada (2019).
Intellectual Property
Patents
1. Fully human antibodies targeting MUC-16/CA125. Canada. Year Issued: 2022
References
Dr. Maruti Uppalapati
Assistant Professor, Department of Pathology and Medical Laboratories, College of Medicine,
University of Saskatchewan, Canada
Email: maruti.uppalapati@usask.ca
Phone: +1-306-490-2068
Dr. Humphrey Fonge
Associate Professor, Department of Medical Imaging, College of Medicine,
University of Saskatchewan, Canada
Email: humphrey.fonge@usask.ca
Phone: +1-306-280-7249
Dr. Ekaterina Dadachova
Fedoruk Chair in Radiopharmacy, Professor of Pharmacy and Nutrition,
University of Saskatchewan, Canada
Email: ekaterina.dadachova@usask.ca
Phone: +1-306-966-5163
Timeline
Post-Doctoral Fellow
University Of Saskatchewan
09.2023 - Current
Quality Assurance Officer - Clinical Trial
University Of Saskatchewan
04.2023 - Current
Teaching Assistant
University Of Saskatchewan
09.2019 - 08.2023
Research Assistant
University Of Saskatchewan
05.2017 - 09.2017
Ph.D. - Health Sciences
University of Saskatchewan
Bachelor of Science - Biochemistry
University of Saskatchewan
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