Filip Vlavcheski
Niagara-on-the-Lake,ON
Summary
Hardworking, highly motivated, and results-driven research scientist with nine years of comprehensive experience in cell biology, metabolic diseases, and the evaluation of bioactive compounds on cellular functions. Extensive skills have been honed in a range of advanced techniques, including human and mammalian cell culture, in vitro and in vivo assays, imaging, and biochemical analyses. This work is underscored by a robust portfolio of eighteen peer-reviewed publications and substantial contributions to the understanding of cell signaling pathways. Proven excellence in designing, overseeing, and executing research projects, along with mentoring both graduate and undergraduate students and trainees. Eager to apply extensive knowledge and skills in a dynamic research setting to drive scientific advancements and deliver transformative discoveries and innovations. Skilled Researcher enthusiastic about supporting advancements in [Area of expertise]. Passionate about increasing knowledge to drive growth and needed improvements. Ready to apply knowledge and [Number] years of experience in research.
Overview
10
10
years of professional experience
1
1
Certification
Work History
Research Associate
Brock University
06.2024 - Current
- Leading research on metabolic diseases and adipocyte function
- Designing and executing experiments related to lipid metabolism and insulin resistance
- Mentoring graduate and undergraduate students in research techniques.
Postdoctoral Research Fellow
Karolinska Institutet
01.2023 - 01.2024
- Investigated lipid handling mechanisms and their impact on cardiometabolic diseases
- Utilized advanced in vitro and in vivo techniques to assess novel therapeutic targets
- Published findings in high-impact journals and presented research at international conferences.
Doctoral Researcher
Brock University
01.2019 - 01.2023
- Conducted extensive research on the effects of carnosic acid on insulin resistance
- Developed and validated in vitro and in vivo models to study adipocyte and muscle cell function
- Authored several high-impact publications and secured funding for research projects.
Master’s Researcher
Brock University
01.2015 - 01.2018
- Investigated rosemary extract's impact on free fatty acid-induced insulin resistance
- Utilized various biochemical and cell culture techniques to support research findings.
Education
Doctor of Philosophy (Ph.D.) - Department of Health Sciences
Brock University
St. Catharines, ONMaster of Science (M.Sc.) - Department of Health Sciences
Brock University
St. Catharines, ONBachelor of Science (B.Sc. Medical Sciences Honors) - Department of Health Sciences
Brock University
St. Catharines, ONPostdoctoral Fellow - Department of Medicine, Lipid handling in health and cardiometabolic disease
Karolinska Institutet
Solna, Sweden01.2024
Skills
- Exceptional understanding of scientific principles and practices: Knowledgeable in core principles of cell and molecular biology, physiology, anatomy, and basic science Expert in the chemical and biochemical interactions of cell culture media, treatments, and other components/reagents
Cell culture: Expertise in various methods and techniques of mammalian and human cell culture, including cell lines used in protein production applications such as CHO, HEK293, and HEK293 mNOD1; production of viral vaccines (ie, MRC-5); adipocytes (3T3-L1 and stromal vascular fraction); muscle cells (L6 and C2C12); and cancer cells Proficient in culturing primary 3D cell cultures obtained from human biopsies, developing treatment formulations, and optimizing cell media components
Biochemical assays: Highly proficient in western blotting, GLUT translocation, glucose and fatty acid uptake and oxidative stress assays, and inflammation assays, ELISA, radioactivity use, PCR, immunocytochemistry, fluorescent labeling,
In vivo techniques: Skilled in tissue harvesting and blood collection, documentation, storage in liquid nitrogen, glucose tolerance tests, ex vivo nutrient uptake assays, immunohistochemistry, and tissue homogenization and processing
Microscopy: Proficient with optical and fluorescence microscopy, including the Nikon A1R Ultra-Fast Spectral Scanning Confocal Microscope
Designing experimental plans: Knowledgeable in planning and designing experiments from conceptualization to execution, either independently or with guidance from a manager Proficient in generating new protocols, writing technical instructions, and drafting reports
Data collection and analysis: Competent in data analysis tools, including ImageJ, FIJI, GraphPad, Microsoft Office, and Cytation5 software Proficient in statistical analysis, demonstrated in publications, and maintaining accurate digital records and written lab notebooks
Data presentation and publication: Comfortable presenting data to large audiences (presented at 9 international and 4 local conferences, as well as numerous smaller seminars/meetings) Skilled in publishing in high-impact journals (published a total of 18 manuscripts, 8 as the first author)
Problem-solving abilities: Able to identify and solve problems by troubleshooting technical, experimental, and logistical issues, efficiently resolving them using creative approaches and techniques
Innovative methods and techniques: Constantly exploring new approaches to update current techniques and knowledge through familiarity with novel technologies and scientific literature
Communication skills: Excellent verbal and written communication skills and the ability to convey scientific results in a concise and accurate manner Experienced in interacting with professors, company representatives, and collaborators in various departments regarding technical issues, products, experimental procedures, and study design
Adherence to safety, training, and quality procedures: Capable of following, implementing, and creating safety regulations in the lab, including handling chemicals, hazardous materials, and radioactivity Proficient in the use of Personal Protective Equipment (PPE), safety glasses, gowns, gloves, lab coats, earplugs, etc
Extensive experience working in a laboratory environment: Able to conduct required duties and tasks, and lift up to 40 lbs as assigned
Excellent organizational skills: Ability to efficiently evaluate and handle multiple projects, prioritize tasks and assignments, as demonstrated by an outstanding publication record
Certification
- General Science Safety Training
- Biosafety Training for CL2 labs
- Radioactivity Training
- Autoclave Training
- Chemical/Gases/Cryogen Transportation
- General Spill Response Training
- WHMIS
- Workplace Violence Training
- X-Ray Safety Training
- Bloodborne Pathogen Safety Training
- COVID-19 Safety
- Analgesia and Anesthesia Certification
Affiliations
- Canadian Society for Exercise Physiology (CSEP)
- International Society for Polyphenol Applications
Selectedpublications
- Vlavcheski, F., MacPherson, R.E.K., Fajardo, V., & Tsiani, E. (2024). Carnosic Acid (CA) Induces a Brown Fat-like Phenotype, Increases Mitochondrial Biogenesis, and Activates AMPK in 3T3-L1 Adipocytes. Biomedicines.
- Den Hartogh, D., Vlavcheski, F., & Tsiani, E. (2023). Muscle Cell Insulin Resistance Is Attenuated by Rosmarinic Acid: Elucidating the Mechanisms Involved. Int J Mol Sci.
- Vlavcheski, F., O’Neill, E.J., Gagacev, F., & Tsiani, E. (2022). Effects of Berberine against Pancreatitis and Pancreatic Cancer. Molecules.
Orcid
0000-0001-9615-1775
Teachingexperience
- Lab Demonstrator - Principles of Human Physiology (HLSC 2P09) and Human Systems Anatomy (HLSC 2F95), Brock University.
- Teaching Assistant - Various courses including Introduction to Health Sciences (HLSC 1F90) and Human Endocrinology (HLSC 3P09).
- Guest Lecturer - Principles of Human Physiology (HLSC 2P09) and Integrated Cardiovascular Pharmacology (4P19).
References
References available upon request.
Timeline
Research Associate
Brock University
06.2024 - Current
Postdoctoral Research Fellow
Karolinska Institutet
01.2023 - 01.2024
Doctoral Researcher
Brock University
01.2019 - 01.2023
Master’s Researcher
Brock University
01.2015 - 01.2018
Doctor of Philosophy (Ph.D.) - Department of Health Sciences
Brock University
Master of Science (M.Sc.) - Department of Health Sciences
Brock University
Bachelor of Science (B.Sc. Medical Sciences Honors) - Department of Health Sciences
Brock University
Postdoctoral Fellow - Department of Medicine, Lipid handling in health and cardiometabolic disease
Karolinska Institutet
- General Science Safety Training
- Biosafety Training for CL2 labs
- Radioactivity Training
- Autoclave Training
- Chemical/Gases/Cryogen Transportation
- General Spill Response Training
- WHMIS
- Workplace Violence Training
- X-Ray Safety Training
- Bloodborne Pathogen Safety Training
- COVID-19 Safety
- Analgesia and Anesthesia Certification
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