Summary
Overview
Work History
Education
Skills
Volunteer Experience
Certification
Languages
Timeline
Generic
Emre Yapici

Emre Yapici

Toronto,ON

Summary

Highly motivated and detail-oriented Cell and Molecular Biology graduate passionate about stem cell and gene therapy, developmental biology, and cancer research. Skilled in a wide range of laboratory techniques and proficient in data analysis. Experienced in designing and conducting research, scientific writing, and communication, with strong analytical thinking and problem-solving abilities. Eager to learn new techniques and technologies while applying my skills and contributing to ongoing research projects.

Overview

2
2
years of professional experience
1
1
Certification

Work History

Molecular Genetics Laboratory Observer

Mersin University
Mersin, Turkey
10.2023 - 12.2023
  • Carcinoma cells are collected from different patients
  • Carcinoma cells are cultured with specific growth factors
  • Cultured carcinoma cells are divided into groups and treated with different concentrations of Retinoic Acid
  • B-actin, YAP, TAZ, and F-actin RNAs are isolated from the treated carcinoma cells
  • RNAs are reverse transcribe into cDNA in a thermal cycler
  • Gene expression analysis performed using RT-PCR with Quantitation Comparative CT
  • (ΔΔCT) values are calculated and statistical analysis performed

Genomics and Proteomics Laboratory

University of Toronto
Toronto, ON
01.2023 - 04.2023
  • Isolate DNA of unknown bacteria strains using Puregene and Monarch Purification Methods, assess the quality and quantity using spectrophotometer and agarose gel electrophoresis.
  • Requantitate DNA using fluorometer, DNA library prep for Illumina sequencing. Perform Quality Control and Trimmomatic on sequences from Illumina using Galaxy platform, align trimmed sequences using Bowtie2, generate consensus sequence of the region of interest using IGV.
  • Identify ORFs using NCBI ORF Finder, Genemark, BLAST, and find the genes corresponding to the ORFs. Find ortholog genes using phylogenetic tree on Mega 11 and write a lab report in the format of a research article.
  • Infiltrate plants with different bacterial pathogens, perform tissue homogenization and phase separation to isolate RNA. Assess the quality and quantity using spectrophotometer and formaldehyde gel electrophoresis.
  • Preprocess RNAseq data using Access database, analyze data using Scatter Plots and PCC. Perform SAM and Cluster analysis on RNAseq data to find differentially expressed genes in response to different bacterial pathogens, and write a lab report in the format of a research article.
  • Spray inoculate plants with different bacterial pathogens, perform tissue homogenization, total protein isolation, and quantitate total protein using Bradford assay.
  • Perform 2D gel electrophoresis to separate proteins, core proteins from gel for Mass spectrometry. Identify modifications, and differentially expressed proteins in response to different bacterial pathogens using MASCOT, domain and motif analysis. Write a lab report in the format of a research article.

Cell and Molecular Biology Laboratory

University of Toronto
Toronto, ON
01.2022 - 04.2022
  • Worked on how knockdown of cec-6 gene affects the expression of PGL-1 and fertility in C.elegans
  • Used techniques like RNAi gene knockdown, designing primers, Gibson Assembly reaction, gel electrophoresis, bacteria transformation, PCR, CRISPR and fluorescent microscopy
  • Worked on the effect of GA on interaction between GID1 receptor and DELLA proteins in A.thaliana
  • Used yeast 2-hybrid technique by transforming plasmids into yeasts, selecting the yeasts by using auxotrophies and screen the results by using reporters
  • Evaluated the results of these 2 works in separate Lab Reports by researching literature and comparing other results

Education

Genetics -

Harvard Medical School - HMX
11.2023

Honours Bachelor of Science - Cell And Molecular Biology Specialist

University of Toronto
06.2023

Skills

  • Laboratory safety, Research and Procedures
  • PCR, CRISPR, Gel electrophoresis, Western Blotting, DNA, RNA, Protein purification, Cell culture and Cell-based assays
  • Molecular cloning, Gene expression, Sequencing
  • Laboratory animal handling
  • Scientific writing and communication
  • Attention to detail
  • Analytical thinking
  • Microsoft Office
  • Teamwork and Collaboration
  • Microscopy and imaging
  • Data analysis

Volunteer Experience

Biology and English Tutor at Outreach Program, 10/2017 - 12/2018

Tarsus American High School, Mersin, Turkey

  • Tutored elementary school children in Biology and English after regular school hours.
  • Conducted engaging and interactive sessions to help students grasp challenging concepts in Biology and English.
  • Facilitated an open dialogue, encouraging students to express areas of difficulty and addressing their questions to ensure comprehension.
  • Developed customized lesson plans and educational activities, promoting a supportive and inclusive learning environment.
  • Worked collaboratively with fellow tutors to enhance the overall impact of the outreach program.

Volunteer at Alzheimer's Assisted Living Facility, 10/2015 - 06/2017

Turkish Alzheimer's Association, Mersin, Turkey

  • Engaged in one-on-one conversations with residents, providing companionship and emotional support.
  • Listened actively to the stories and experiences shared by individuals with Alzheimer's, fostering a sense of connection.
  • Attend group activities, games, and other recreational programs to stimulate mental well-being.
  • Collaborated with fellow volunteers and healthcare professionals to create a caring and supportive environment.
  • Contributed to the overall mission of the association to enhance the quality of life for individuals with Alzheimer's.

Certification

  • Certificate of Laboratory Animal Usage and Handling - 12/2023

(MEUSEM/HADYEK)

  • Certificate of Cell Culture Basics - 11/2023

(Thermo Fisher Scientific - Gibco Virtual Lab)

  • Certificate of Achievement in Gene Therapy - 11/2023
  • Certificate of Achievement in Genetic Testing and Sequencing Technologies - 11/2023
  • Certificate of Achievement in Cancer Genomics and Precision Oncology - 11/2023

(Harvard Medical School - HMX)

Languages

  • Turkish: Native
  • English: Fluent
  • French: Elementary

Timeline

Molecular Genetics Laboratory Observer

Mersin University
10.2023 - 12.2023

Genomics and Proteomics Laboratory

University of Toronto
01.2023 - 04.2023

Cell and Molecular Biology Laboratory

University of Toronto
01.2022 - 04.2022

Genetics -

Harvard Medical School - HMX

Honours Bachelor of Science - Cell And Molecular Biology Specialist

University of Toronto

Similar Profiles

CREATE PROFILE
Emre Yapici