DANIEL RAMOS
Upland,CA
Summary
Experienced scientist with over 7 years of practical experience, focusing in biotechnology. Knowledgeable about creating and validating cutting-edge medical equipment and diagnostic techniques based on nanotechnology, molecular biology, and biochemistry. Eager to take the lead on innovative product initiatives and actively participate in projects. The goal is to lead the way in creating innovative products that give companies a competitive edge, directing enthusiasm for turning scientific discoveries into real, beneficial medical developments.
Overview
8
8
years of professional experience
Work History
Research Assistant
Keck Graduate Institute
09.2023 - Current
- Implemented a standardized multiplex RT-PCR protocol utilizing 3 distinct primer sets for the identification of AVV.
- Conducted RNA isolation and cDNA production in AVV bioprocessing for quality control assays and safety.
Research Assistant
Keck Graduate Institute
09.2022 - 05.2023
- Completed production of 9 variants within 6-month timeframe
- Increased purification yield by 40% using AKTA chromatography and tangential flow filtration techniques
- Evaluated 9 variants of RBD protein through dot blot, northern blot, western blot, BCA assay, and biolayer interferometry (BLA).
Lab Technician I - CRISPR
QC
05.2022 - 08.2022
- Standardized assays for assessing sgRNA interaction with CAS9 activity using graphene sensor field-effect electronic (FET)
- Used automated robotic system and laboratory information management System (LIMS) for daily reporting.
Research Assistant
Keck Graduate Institute
09.2021 - 05.2022
- Ensured quality assurance by maintaining 98% consistency rate across 100 chips
- Achieved 95% immobilization efficiency of CAS protein using layer-by-layer technique
- Employed GraphPad software to ensure precise documented results, resulting in 40% reduction in analysis time.
Research Associate
I - Univ. Peruana Cayetano Heredia
02.2016 - 08.2021
- Developed, and validated electrochemical genosensor with 200 samples for detection of Mycobacterium tuberculosis
- Characterized 3 types of biosensor surfaces using electrochemical,atomic force microscopy,and Raman spectroscopy techniques.
Education
Master of Science - Business And Science
Keck Graduate Institute
Claremont05.2023
Bachelor of Science - Biology
Universidad Nacional Pedro Ruiz Gallo
Perú03.2015
Skills
- SKILLS AND ABILITIES
- Bacterial Culture
- Bioconjugation
- ELISA
- Dot Blot
- Electrophoresis
- Biolayer interferometry
- Surface Plasmonic Resonance
- Python, R
- GraphPad
- Teamwork and Collaboration
- Microsoft Office
- Time Management
- Problem-Solving
- Data Collection
Timeline
Research Assistant
Keck Graduate Institute
09.2023 - Current
Research Assistant
Keck Graduate Institute
09.2022 - 05.2023
Lab Technician I - CRISPR
QC
05.2022 - 08.2022
Research Assistant
Keck Graduate Institute
09.2021 - 05.2022
Research Associate
I - Univ. Peruana Cayetano Heredia
02.2016 - 08.2021
Master of Science - Business And Science
Keck Graduate Institute
Bachelor of Science - Biology
Universidad Nacional Pedro Ruiz Gallo
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