Cheng LONG

Cheng is a lecturer on improving reproductive performance in laying roosters, where he is working to illuminate the relationship between gut microbiota and testes (gut-testes axis). He is interested in the role of the gut microbiome in nutrition and health. Cheng did his Ph.D. at Maastricht University in the Netherlands, Dietary fibre plays an important role in maintaining gut health and preventing diseases. Therefore, he worked on developing new pre/probiotics and improving fiber digestibility. He also worked on developing a molecular biology method to screen carbohydrate constitution, a high throughput method to recognize cell-wall polysaccharides using carbohydrate-binding modules (CBM). He is also interested in bioinformatics, and he spends much of his leisure time self-learning Linux, Python, and R programs, and also some applied bioinformatics courses.

Cheng LONG, male, 07/19/1990

• Exploring the effect and molecular mechanism of pyrroloquinoline quinone on improving the blood-testis barrier function of aged roosters based on tight junction protein expression, National Natural Science Foundation of China, ￥300,000, 2024-2026
• The mechanism of chlorogenic acid-mediated gut microbiota metabolites regulating the rooster spermatogenesis, Beijing Municipal Education Commission, ￥150,000, 2023-2025
• The effect of pyrroloquinoline quinone and selenomethionine on the rooster’s reproduction performance, Beijing University of Agriculture, ￥100,000, 2022-2023

• Lecturer, Beijing University of Agriculture, Beijing, CN, 06/2021, Present, Cheng is a lecturer and working on improving reproductive performance in breeding roosters via modulating gut microbiota (gut-testes axis). To unfold these, muti-omics (transcriptome, metabolome, and 16S rRNA sequencing) were applied to investigate how the gut microbiota (metabolites) modulate testes’ sperm production. Meantime, wet-lab techniques (cell culture (stem spermatogonial cells), qRT-PCR, Western Blot, flow cytometry, confocal Laser Scanning Microscope, etc ) were used to explore inside mechanisms.
• Ph.D. Researcher, Maastricht University, Maastricht, NL, 09/2016, 11/2020, Cheng did his Ph.D. at the University of Maastricht, supervised by Prof. Dr. Koen Venema, and the title of my project is "Improving intestinal degradation of recalcitrant fibers in pigs". We have been using multi-disciplinary approaches, such as in vitro models, next-generation sequencing, molecular biological technique, and food chemistry methodologies, to unfold the mechanism of fiber degradation by gut microbiota in pigs.
• Wageningen University, Wageningen, NL, 06/2019, 08/2020, Ileal cannulated pigs were used to ferment the fibers which were performed in Animal Nutrition Group; monosaccharide constitution was analyzed in the Laboratory of Food Chemistry, where gas chromatography (GC), high-performance size-exclusion chromatography (HPSEC), and high-performance anion-exchange chromatography (HPAEC) were used.

• Long C, Qi XL, Venema K, Chemical and nutritional characteristics, and microbial degradation of rapeseed meal recalcitrant carbohydrates: A review, Frontiers in Nutrition, 2022, 6.58, Q1
• Long C, Zhu GY, Sheng XH, Xing K, Venema K, Wang XG, Xiao LF, Guo Y, Ni HM, Zhu NH, Qi XL, Dietary supplementation with selenomethionine enhances antioxidant capacity and selenoprotein gene expression in layer breeder roosters, Poultry Science, 2022, 4.014, Q1
• Long C, Wang Z, Guo Y, Sheng X, Xing K, Ni H, Wang X, Xiao L, Qi X, Research Note: Dietary supplementation with pyrroloquinoline quinone disodium (PQQ. Na2) improves oxidative status and semen quality in aging layer breeder roosters, Poultry Science, 2022, 4.014, Q1
• Long C, Rösch C, de Vries S, Schols HA, Venema K, Cellulase and alkaline treatment improves intestinal microbial degradation of recalcitrant fibres of rapeseed meal in pigs, Journal of Agricultural and Food Chemistry, 2020, 5.895, Q1
• Long C, de Vries S, Venema K, Differently pre-treated rapeseed meals affect in vitro swine gut Microbiota composition, Frontiers in Microbiology, 2020, 6.064, Q1
• Long C, Venema K, Pretreatment of rapeseed meal increases its recalcitrant fibre fermentation and alters the microbial community in an in vitro model of swine large intestine, Frontiers in Microbiology, 2020, 6.064, Q1
• Long C, de Vries S, Venema K, Polysaccharides source alters molecular ecological network interaction, functional profile, and short-chain fatty acid production in porcine gut microbiota, Beneficial Microbes, 2020, 5.050, Q2
• Long C, Wang J, Wu SG, Zhang HJ, Qi GH, Effects of dietary rapeseed meal supplementation on cecal microbiota in laying hens with different flavin-containing monooxygenase 3 genotypes, Poultry Science, 2017, 4.014, Q1
• Long C, de Vries S, Schols HA, Venema K, Effect of different sources of starch on composition and activity of a pig microbiota in a validated, dynamic in vitro model of the colon, 14th International Symposium on Digestive Physiology of Pigs (DPP2018), 2018
• Long C, Venema K, Chemical and nutritional characteristics, and microbial degradation of rapeseed meal recalcitrant carbohydrates, Animal Nutrition, Under review
• Long C, Venema K, High-throughput screening of structures of plant cell wall polysaccharides using a novel method, Prepared for publication
• Long C, Chen L, Wang J, Wu SG, Zhang HJ, Yue HY, Qi GH, Effects of Dietary Canola Meal Level on Performance, Egg Quality, Trimethylamine Content in Egg Yolk of Flavin-Containing Monooxygenases 3 Genotype Laying Hens, Chinese Journal of Animal Nutrition, 2016, 28, 6, 1687-1695
• Long C, Wang J, Wu SG, Zhang HJ, Yue HY, Qi GH, Influence of Feed Factors on Egg Flavor and the Measures for Flavor Improvement, Chinese Journal of Animal Nutrition, 2015, 27, 2, 352-358
• Wang J, Long C, Zhang HJ, Zhang Y, Wang H, Yue HY, Wang X, Wu SG, Qi GH, Genetic variant in Flavin-containing monooxygenase 3 alters lipid metabolism in laying hens in a diet-specific manner, International Journal of Biological Sciences, 2016, 12, 11, 1382
• Wang J, Zhang HJ, Xu L, Long C, Samuel KG, Yue HY, Sun LL, Wu SG, Qi GH, Dietary supplementation of pyrroloquinoline quinone disodium protects against oxidative stress and liver damage in laying hens fed an oxidized sunflower oil-added diet, Animal, 2016, 10, 7, 1129-1136
• Wang J, Zhang HJ, Samuel KG, Long C, Wu SG, Yue HY, Sun LL, Qi GH, Effects of dietary pyrroloquinoline quinone disodium on growth, carcass characteristics, redox status, and mitochondria metabolism in broilers, Poultry Science, 2015, 94, 2, 215-225

• 14th International Symposium Digestive Physiology of Pigs - August 2018, Brisbane
• The 7th Beneficial Microbes Conference - November 2018, Amsterdam
• The 6th Beneficial Microbes Conference - October 2017, Amsterdam
• The 19th Gutday - November 2017, Venlo
• The 20th Gutday - December 2018, Middleburg