Brindhi Amalraj

• • Co-founded a one-step serum separation technology from blood samples to support at-home preliminary breast cancer testing for women.
• • Spearheaded the research and development team, by designing and executing immunoassays for the CA 15-3 antigen for the successful validation of prototypes.
• • Formulated and implemented comprehensive standard operating procedures while providing training to undergraduate interns for accurate result collection.

• Demonstrated expertise in delivering pragmatic solutions to client challenges by analyzing data, crafting comprehensive reports, and articulating findings to both clients and senior leadership.

• Pioneered the coordination of a global community of problem-solvers, facilitating connections among subject matter experts in government and the public sector in different countries to support cross-functional teams and develop thought leadership on various pressing social sector issues.

• Successfully executed 7 projects in different social sector areas including supply chain and material management, performance framework, cost modelling and continuous improvement by applying BxT, SAFe Agile, and Lean project management methodologies, ensuring efficient and collaborative project delivery.

• Identified critical contributors to cellular signalling mechanisms affecting self-incompatibility in Brassica napus.

• Proficient in RT-PCR, ddPCR, CRISPR-Cas9 vector design and cloning, and a range of protein characterization techniques including culture, isolation, purification, SDS-Page, and western blot analysis.

• Conceived and conducted experiments aimed at revealing previously unidentified components in the molecular processes governing vascular development.

• Identified 89 RNA targets in the vascular development pathway and confirmed the role of 4 targets through targeted mutagenesis, RNA sequencing, fluorescence imaging and phenotypical analysis.

• Acquired three years of practical expertise in plasmid vector and construct design, CRISPR-Cas9 techniques, DNA and RNA isolation, purification, sequencing, and analysis, as well as proficiency in PCR, RT-PCR, and qPCR.

• Conducted research into the influence of the UmuC gene on E. coli growth dynamics and examined the impact of ethyl methane sulfonate (EMS) on the growth of UmuC mutants.

• Trained in mammalian cell culture and maintenance, protein isolation, plasmid construction, and adept at safely handling ethyl methylsulfonate mutagen.