YU HUANG

Evaluating High-Sensitivity C-Reactive Protein as an Independent Risk Factor for Cardiovascular Disease

• Collect blood test and physical examination data of patients with cardiovascular disease and healthy individuals from the UK Biobank.
• Import data into the SPSS (Statistical Package for the Social Sciences) program for data analysis and graphical analysis.

Antibiotic Produced from Streptomyces that isolated from soil and Chloramphenicol sensitive to Gram-positive bacteria

• Cultivating Streptomyces isolated from soil.
• Comparing Streptomyces in the environment of three different indicator bacteria.
• Observing the sensitivity of chloramphenicol to gram-positive and gram-negative bacteria.

The effect of Doxorubicin on expression of the cell cycle regulator, p21, in breast cancer (MCF7) cells

• Lysed MCF7 breast cancer cells.
• Primer design
• Isolate RNA and purif
• Reverse transcribe to cDNA
• Use real-time PCR with sybr green.

Biobrick and applied to the fliC-containing strain to measure promoter strength of the reporter gene. The promoter intensity was then measured based on the fluorescence level of the sub-GFP

• Incubate E.coli DS941 Z1 strand with different plasmids
• Competent cells for transformatio
• Using selected enzymes for performing restriction digestions to cut DNA
• Measure the green fluorescence protein expression.

The mutation of Phototropin2 Light, Oxygen, and Voltage protein is the future of optogenetic tool

• Using western blot to determine the protein size
• Cell transformation
• Making molecular and protein structure graphs by LOV-based Optogenetic Tools
• Using PyMOL to Visualise Protein Structures.

Internship experience

• Intern in Jiangmen Central Hospital molecular biology laboratory.

Work experience

• Work in the Enhanced Care Clinic as a medical assistant.

School cultural evening volunteer activity organization

• Organize student participation in volunteer activities and determine the division of labor.

Lab group leader

• Organize the experiment outline, assign tasks, and conduct final confirmation checks.

• Molecular biology techniques: Molecular biology techniques: PCR, qPCR, RT-PCR, western blot, RNA isolation, extraction, primer design, DNA cloning, gel electrophoresis, DNA extraction and purification, next-generation sequencing, CRISPR-Cas9 Gene Editing
• Microsoft offices
• Python, Adobe, Photoshop, SPSS
• Language: Fluent in English, Mandarin, Cantonese